Transposition of a fungal mite through the action of a Tc1-like transposase

M. Dufresne, A. Hua-Van, H. Abd el Wahab, S. Ben M'Barek, G.H.J. Kema, M.J. Daboussi

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    32 Citations (Scopus)

    Abstract

    The mimp1 element previously identified in the ascomycete fungus Fusarium oxysporum has hallmarks of miniature inverted-repeat transposable elements (MITEs): short size, terminal inverted repeats (TIRs), structural homogeneity, and a stable secondary structure. Since mimp1 has no coding capacity, its mobilization requires a transposase-encoding element. On the basis of the similarity of TIRs and target-site preference with the autonomous Tcl-like element impala, together with a correlated distribution of both elements among the Fusarium genus, we investigated the ability of mimp1 to jump upon expression of the impala transposase provided in trans. Under these conditions, we present evidence that mimp1 transposes by a cut-and-paste mechanism into TA dinucleoticles, which are duplicated upon insertion. Our results also show that mimp1 reinserts very frequently in genic regions for at least one-third of the cases. We also show that the mimp1/impala, double-component system is fully functional in the heterologous species F. graminearum, allowing the development of a highly efficient tool for gene tagging in filamentous fungi.
    Original languageEnglish
    Pages (from-to)441-452
    JournalGenetics
    Volume175
    DOIs
    Publication statusPublished - 2007

    Keywords

    • fusarium-oxysporum
    • arabidopsis-thaliana
    • dna transposon
    • caenorhabditis-elegans
    • aspergillus-nidulans
    • neurospora-crassa
    • genome sequence
    • emigrant family
    • rice
    • genes

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  • Cite this

    Dufresne, M., Hua-Van, A., Abd el Wahab, H., Ben M'Barek, S., Kema, G. H. J., & Daboussi, M. J. (2007). Transposition of a fungal mite through the action of a Tc1-like transposase. Genetics, 175, 441-452. https://doi.org/10.1534/genetics.106.064360