Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria

Elena V. Eremeeva; Willem J.H. Van Berkel; Eugene S. Vysotski

doi:10.1002/1873-3468.12052

Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria

Elena V. Eremeeva, Willem J.H. Van Berkel, Eugene S. Vysotski^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca²⁺-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.

Original language	English
Pages (from-to)	307-316
Journal	FEBS Letters
Volume	590
Issue number	3
DOIs	https://doi.org/10.1002/1873-3468.12052
Publication status	Published - 2016

Keywords

aequorin
bioluminescence
coelenterazine
FRET
obelin
protein-protein interaction

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10.1002/1873-3468.12052

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title = "Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria",

abstract = "Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca2+-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.",

keywords = "aequorin, bioluminescence, coelenterazine, FRET, obelin, protein-protein interaction",

author = "Eremeeva, {Elena V.} and {Van Berkel}, {Willem J.H.} and Vysotski, {Eugene S.}",

year = "2016",

doi = "10.1002/1873-3468.12052",

language = "English",

volume = "590",

pages = "307--316",

journal = "FEBS Letters",

issn = "0014-5793",

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TY - JOUR

T1 - Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria

AU - Eremeeva, Elena V.

AU - Van Berkel, Willem J.H.

AU - Vysotski, Eugene S.

PY - 2016

Y1 - 2016

N2 - Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca2+-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.

AB - Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca2+-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.

KW - aequorin

KW - bioluminescence

KW - coelenterazine

KW - FRET

KW - obelin

KW - protein-protein interaction

U2 - 10.1002/1873-3468.12052

DO - 10.1002/1873-3468.12052

M3 - Article

AN - SCOPUS:84959116737

SN - 0014-5793

VL - 590

SP - 307

EP - 316

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria

Abstract

Keywords

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