Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria

Elena V. Eremeeva, Willem J.H. Van Berkel, Eugene S. Vysotski*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca2+-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.

Original languageEnglish
Pages (from-to)307-316
JournalFEBS Letters
Volume590
Issue number3
DOIs
Publication statusPublished - 2016

Keywords

  • aequorin
  • bioluminescence
  • coelenterazine
  • FRET
  • obelin
  • protein-protein interaction

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