Transient heterogeneity in extracellular protease production by Bacillus subtilis

Jan Willem Veening, Oleg A. Igoshin, Robyn T. Eijlander, Reindert Nijland, Leendert W. Hamoen, Oscar P. Kuipers

Research output: Contribution to journalArticleAcademicpeer-review

115 Citations (Scopus)

Abstract

The most sophisticated survival strategy Bacillus subtilis employs is the differentiation of a subpopulation of cells into highly resistant endospores. To examine the expression patterns of non-sporulating cells within heterogeneous populations, we used buoyant density centrifugation to separate vegetative cells from endospore-containing cells and compared the transcriptome profiles of both subpopulations. This demonstrated the differential expression of various regulons. Subsequent single-cell analyses using promoter-gfp fusions confirmed our microarray results. Surprisingly, only part of the vegetative subpopulation highly and transiently expresses genes encoding the extracellular proteases Bpr (bacillopeptidase) and AprE (subtilisin), both of which are under the control of the DegU transcriptional regulator. As these proteases and their degradation products freely diffuse within the liquid growth medium, all cells within the clonal population are expected to benefit from their activities, suggesting that B. subtilis employs cooperative or even altruistic behavior. To unravel the mechanisms by which protease production heterogeneity within the non-sporulating subpopulation is established, we performed a series of genetic experiments combined with mathematical modeling. Simulations with our model yield valuable insights into how population heterogeneity may arise by the relatively long and variable response times within the DegU autoactivating pathway.

Original languageEnglish
Article number184
JournalMolecular Systems Biology
Volume4
Issue number1
DOIs
Publication statusPublished - 22 Apr 2008
Externally publishedYes

Keywords

  • Bacillus subtilis
  • Bistability
  • DegU
  • Modeling
  • Sporulation

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