Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Michael J. Stephenson, James Reed, B. Brouwer, Anne Osbourn

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Transient heterologous expression of biosynthetic enzymes in Nicotiana benthamiana leaves can divert endogenous supplies of 2,3-oxidosqualene towards the production of new high-value triterpene products. Herein is described a detailed protocol for rapid (5 days) preparative-scale production of triterpenes and analogs utilizing this powerful plant-based platform.
Original languageEnglish
Article numbere58169
JournalJournal of Visualized Experiments
DOIs
Publication statusPublished - 2018
Externally publishedYes

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