Transgenic Spodoptera exigua: possibilities for their use

L.J.M. Gerritsen, J.H. Visser, M.A. Jongsma

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

    Abstract

    Transgenic Spodoptera exigua are being developed by microinjection of a piggyBac vector. The vector expresses green fluorescent protein (GFP) under the control of the actin promoter. Forty percent of the first-instar larvae that hatched from the injected eggs were green fluorescent. However, after backcrossing none of the G1 first-instar larvae was fluorescent and a transgenic line could not be established. Several possibilities for the use of transgenic insects are discussed
    Original languageEnglish
    Title of host publicationProceedings of the section Experimental and Applied Entomology of the Netherlands Entomological Society (NEV)
    Place of PublicationAmsterdam
    PublisherNederlandse Entomologische Vereniging (NEV)
    Pages127-129
    Volume13
    ISBN (Print)907191223X
    Publication statusPublished - 2002
    Event13th annual meeting of entomologists in The Netherlands -
    Duration: 15 Aug 200215 Aug 2002

    Conference

    Conference13th annual meeting of entomologists in The Netherlands
    Period15/08/0215/08/02

    Fingerprint

    Spodoptera exigua
    instars
    transgenic insects
    genetically modified organisms
    larvae
    backcrossing
    green fluorescent protein
    actin
    promoter regions

    Cite this

    Gerritsen, L. J. M., Visser, J. H., & Jongsma, M. A. (2002). Transgenic Spodoptera exigua: possibilities for their use. In Proceedings of the section Experimental and Applied Entomology of the Netherlands Entomological Society (NEV) (Vol. 13, pp. 127-129). Amsterdam: Nederlandse Entomologische Vereniging (NEV).
    Gerritsen, L.J.M. ; Visser, J.H. ; Jongsma, M.A. / Transgenic Spodoptera exigua: possibilities for their use. Proceedings of the section Experimental and Applied Entomology of the Netherlands Entomological Society (NEV). Vol. 13 Amsterdam : Nederlandse Entomologische Vereniging (NEV), 2002. pp. 127-129
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    abstract = "Transgenic Spodoptera exigua are being developed by microinjection of a piggyBac vector. The vector expresses green fluorescent protein (GFP) under the control of the actin promoter. Forty percent of the first-instar larvae that hatched from the injected eggs were green fluorescent. However, after backcrossing none of the G1 first-instar larvae was fluorescent and a transgenic line could not be established. Several possibilities for the use of transgenic insects are discussed",
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    Gerritsen, LJM, Visser, JH & Jongsma, MA 2002, Transgenic Spodoptera exigua: possibilities for their use. in Proceedings of the section Experimental and Applied Entomology of the Netherlands Entomological Society (NEV). vol. 13, Nederlandse Entomologische Vereniging (NEV), Amsterdam, pp. 127-129, 13th annual meeting of entomologists in The Netherlands, 15/08/02.

    Transgenic Spodoptera exigua: possibilities for their use. / Gerritsen, L.J.M.; Visser, J.H.; Jongsma, M.A.

    Proceedings of the section Experimental and Applied Entomology of the Netherlands Entomological Society (NEV). Vol. 13 Amsterdam : Nederlandse Entomologische Vereniging (NEV), 2002. p. 127-129.

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

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    AU - Jongsma, M.A.

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    N2 - Transgenic Spodoptera exigua are being developed by microinjection of a piggyBac vector. The vector expresses green fluorescent protein (GFP) under the control of the actin promoter. Forty percent of the first-instar larvae that hatched from the injected eggs were green fluorescent. However, after backcrossing none of the G1 first-instar larvae was fluorescent and a transgenic line could not be established. Several possibilities for the use of transgenic insects are discussed

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    Gerritsen LJM, Visser JH, Jongsma MA. Transgenic Spodoptera exigua: possibilities for their use. In Proceedings of the section Experimental and Applied Entomology of the Netherlands Entomological Society (NEV). Vol. 13. Amsterdam: Nederlandse Entomologische Vereniging (NEV). 2002. p. 127-129