Transgenic modification of potato pectic polysaccharides also affects type and level of cell wall xyloglucan

Jie Hong Huang, Rui Jiang, Anne Kortstee, Dianka C.T. Dees, Luisa M. Trindade, Harry Gruppen, Henk A. Schols*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

BACKGROUND: Genes encoding pectic enzymes were introduced into wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing β-galactosidase (β-Gal-14) or rhamnogalacturonan lyase (RGL-18). Pectic polysaccharides from the β-Gal-14 transgenic line exhibited rhamnogalacturonan-I structural elements with shorter galactan side chains, whereas the RGL-18 transgenic line had less rhamnogalacturonan-I structures than Karnico. Xyloglucan in primary cell walls interacts with pectin and other cell wall polysaccharides and controls cell growth. RESULTS: Xyloglucan extracts from transgenic lines had different levels of monosaccharides compared to wild-type. Most XXGG-type xyloglucans from Karnico and RGL-18 alkali-extractable extracts predominantly consisted of XXGG and XSGG building blocks. Karnico and RGL-18 4molL-1 extracts had small proportions of the XXXG-type xyloglucan, whereas β-Gal-14 extracts also contained the XXXG-type xyloglucan. The peak ratios of XSGG/XXGG were 1.9, 2.4 and 1.1 for 4molL-1 extracts of Karnico, RGL-18 and β-Gal-14 lines, respectively. CONCLUSION: After transgenic modification on pectin, the xyloglucan building blocks may have been changed. The β-Gal-14 lines mostly present XXXG-type repeating units instead of the XXGG-type in 4molL-1 extracts. The ratio of XSGG/XXGG repeating units also changed, indicating that the transgenic modification of pectin altered xyloglucan structure during plant development.
Original languageEnglish
Pages (from-to)3240-3248
JournalJournal of the Science of Food and Agriculture
Volume97
Issue number10
DOIs
Publication statusPublished - 2017

Fingerprint

xyloglucans
Solanum tuberosum
Cell Wall
Polysaccharides
polysaccharides
cell walls
genetically modified organisms
potatoes
extracts
pectins
Galactans
Galactosidases
galactosidases
galactans
Lyases
Plant Development
Monosaccharides
lyases
Alkalies
monosaccharides

Keywords

  • Cell wall polysaccharides
  • Non-targeted modification
  • Oligomers
  • Solanum tuberosum

Cite this

@article{e39fd0c7b357407293d162663832ae79,
title = "Transgenic modification of potato pectic polysaccharides also affects type and level of cell wall xyloglucan",
abstract = "BACKGROUND: Genes encoding pectic enzymes were introduced into wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing β-galactosidase (β-Gal-14) or rhamnogalacturonan lyase (RGL-18). Pectic polysaccharides from the β-Gal-14 transgenic line exhibited rhamnogalacturonan-I structural elements with shorter galactan side chains, whereas the RGL-18 transgenic line had less rhamnogalacturonan-I structures than Karnico. Xyloglucan in primary cell walls interacts with pectin and other cell wall polysaccharides and controls cell growth. RESULTS: Xyloglucan extracts from transgenic lines had different levels of monosaccharides compared to wild-type. Most XXGG-type xyloglucans from Karnico and RGL-18 alkali-extractable extracts predominantly consisted of XXGG and XSGG building blocks. Karnico and RGL-18 4molL-1 extracts had small proportions of the XXXG-type xyloglucan, whereas β-Gal-14 extracts also contained the XXXG-type xyloglucan. The peak ratios of XSGG/XXGG were 1.9, 2.4 and 1.1 for 4molL-1 extracts of Karnico, RGL-18 and β-Gal-14 lines, respectively. CONCLUSION: After transgenic modification on pectin, the xyloglucan building blocks may have been changed. The β-Gal-14 lines mostly present XXXG-type repeating units instead of the XXGG-type in 4molL-1 extracts. The ratio of XSGG/XXGG repeating units also changed, indicating that the transgenic modification of pectin altered xyloglucan structure during plant development.",
keywords = "Cell wall polysaccharides, Non-targeted modification, Oligomers, Solanum tuberosum",
author = "Huang, {Jie Hong} and Rui Jiang and Anne Kortstee and Dees, {Dianka C.T.} and Trindade, {Luisa M.} and Harry Gruppen and Schols, {Henk A.}",
year = "2017",
doi = "10.1002/jsfa.8172",
language = "English",
volume = "97",
pages = "3240--3248",
journal = "Journal of the Science of Food and Agriculture",
issn = "0022-5142",
publisher = "Wiley",
number = "10",

}

Transgenic modification of potato pectic polysaccharides also affects type and level of cell wall xyloglucan. / Huang, Jie Hong; Jiang, Rui; Kortstee, Anne; Dees, Dianka C.T.; Trindade, Luisa M.; Gruppen, Harry; Schols, Henk A.

In: Journal of the Science of Food and Agriculture, Vol. 97, No. 10, 2017, p. 3240-3248.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Transgenic modification of potato pectic polysaccharides also affects type and level of cell wall xyloglucan

AU - Huang, Jie Hong

AU - Jiang, Rui

AU - Kortstee, Anne

AU - Dees, Dianka C.T.

AU - Trindade, Luisa M.

AU - Gruppen, Harry

AU - Schols, Henk A.

PY - 2017

Y1 - 2017

N2 - BACKGROUND: Genes encoding pectic enzymes were introduced into wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing β-galactosidase (β-Gal-14) or rhamnogalacturonan lyase (RGL-18). Pectic polysaccharides from the β-Gal-14 transgenic line exhibited rhamnogalacturonan-I structural elements with shorter galactan side chains, whereas the RGL-18 transgenic line had less rhamnogalacturonan-I structures than Karnico. Xyloglucan in primary cell walls interacts with pectin and other cell wall polysaccharides and controls cell growth. RESULTS: Xyloglucan extracts from transgenic lines had different levels of monosaccharides compared to wild-type. Most XXGG-type xyloglucans from Karnico and RGL-18 alkali-extractable extracts predominantly consisted of XXGG and XSGG building blocks. Karnico and RGL-18 4molL-1 extracts had small proportions of the XXXG-type xyloglucan, whereas β-Gal-14 extracts also contained the XXXG-type xyloglucan. The peak ratios of XSGG/XXGG were 1.9, 2.4 and 1.1 for 4molL-1 extracts of Karnico, RGL-18 and β-Gal-14 lines, respectively. CONCLUSION: After transgenic modification on pectin, the xyloglucan building blocks may have been changed. The β-Gal-14 lines mostly present XXXG-type repeating units instead of the XXGG-type in 4molL-1 extracts. The ratio of XSGG/XXGG repeating units also changed, indicating that the transgenic modification of pectin altered xyloglucan structure during plant development.

AB - BACKGROUND: Genes encoding pectic enzymes were introduced into wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing β-galactosidase (β-Gal-14) or rhamnogalacturonan lyase (RGL-18). Pectic polysaccharides from the β-Gal-14 transgenic line exhibited rhamnogalacturonan-I structural elements with shorter galactan side chains, whereas the RGL-18 transgenic line had less rhamnogalacturonan-I structures than Karnico. Xyloglucan in primary cell walls interacts with pectin and other cell wall polysaccharides and controls cell growth. RESULTS: Xyloglucan extracts from transgenic lines had different levels of monosaccharides compared to wild-type. Most XXGG-type xyloglucans from Karnico and RGL-18 alkali-extractable extracts predominantly consisted of XXGG and XSGG building blocks. Karnico and RGL-18 4molL-1 extracts had small proportions of the XXXG-type xyloglucan, whereas β-Gal-14 extracts also contained the XXXG-type xyloglucan. The peak ratios of XSGG/XXGG were 1.9, 2.4 and 1.1 for 4molL-1 extracts of Karnico, RGL-18 and β-Gal-14 lines, respectively. CONCLUSION: After transgenic modification on pectin, the xyloglucan building blocks may have been changed. The β-Gal-14 lines mostly present XXXG-type repeating units instead of the XXGG-type in 4molL-1 extracts. The ratio of XSGG/XXGG repeating units also changed, indicating that the transgenic modification of pectin altered xyloglucan structure during plant development.

KW - Cell wall polysaccharides

KW - Non-targeted modification

KW - Oligomers

KW - Solanum tuberosum

U2 - 10.1002/jsfa.8172

DO - 10.1002/jsfa.8172

M3 - Article

VL - 97

SP - 3240

EP - 3248

JO - Journal of the Science of Food and Agriculture

JF - Journal of the Science of Food and Agriculture

SN - 0022-5142

IS - 10

ER -