Transgenic caraway, Carum carvi L.: a model species for metabolic engineering

F.A. Krens, L.C.P. Keizer, I.E.M. Capel

    Research output: Contribution to journalArticleAcademicpeer-review

    12 Citations (Scopus)

    Abstract

    Regeneration in caraway was obtained via two different routes. Hypocotyls showed delayed shoot formation after a callus phase and at relatively low frequencies. In contrast, high-frequency, direct regeneration occurred when cotyledonary node explants were used. Transient expression of #-glucuronidase was monitored after inoculation of both explant types with Agrobacterium tumefaciens AGL0(pMOG410). Gene transfer was more efficient when using cotyledonary node explants. This explant type also proved to be the best for stable transformation resulting in transgenic plants. Several parameters determining regeneration and transformation efficiency were tested. The percentage of explants giving one to numerous transgenic plants could be as high as 13%. This system for the rapid production of many transgenic caraway plants opens up possibilities for studying metabolic engineering with this crop.
    Original languageEnglish
    Pages (from-to)39-43
    JournalPlant Cell Reports
    Volume17
    Issue number1
    DOIs
    Publication statusPublished - 1997

    Fingerprint

    Carum carvi
    caraway
    metabolic engineering
    explants
    genetically modified organisms
    transgenic plants
    Agrobacterium radiobacter
    gene transfer
    hypocotyls
    callus
    shoots
    crops

    Cite this

    @article{f27e23d6da6348a7abd66a9d21a5af5f,
    title = "Transgenic caraway, Carum carvi L.: a model species for metabolic engineering",
    abstract = "Regeneration in caraway was obtained via two different routes. Hypocotyls showed delayed shoot formation after a callus phase and at relatively low frequencies. In contrast, high-frequency, direct regeneration occurred when cotyledonary node explants were used. Transient expression of #-glucuronidase was monitored after inoculation of both explant types with Agrobacterium tumefaciens AGL0(pMOG410). Gene transfer was more efficient when using cotyledonary node explants. This explant type also proved to be the best for stable transformation resulting in transgenic plants. Several parameters determining regeneration and transformation efficiency were tested. The percentage of explants giving one to numerous transgenic plants could be as high as 13{\%}. This system for the rapid production of many transgenic caraway plants opens up possibilities for studying metabolic engineering with this crop.",
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    year = "1997",
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    pages = "39--43",
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    Transgenic caraway, Carum carvi L.: a model species for metabolic engineering. / Krens, F.A.; Keizer, L.C.P.; Capel, I.E.M.

    In: Plant Cell Reports, Vol. 17, No. 1, 1997, p. 39-43.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Transgenic caraway, Carum carvi L.: a model species for metabolic engineering

    AU - Krens, F.A.

    AU - Keizer, L.C.P.

    AU - Capel, I.E.M.

    PY - 1997

    Y1 - 1997

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