Transformation of the cultivated mushroom Agaricus bisporus (Lange) using T-DNA from Agrobacterium tumefaciens.

T.S.P. Mikosch, B. Lavrijssen, L.J.L.D. van Griensven

    Research output: Contribution to journalArticleAcademicpeer-review

    55 Citations (Scopus)

    Abstract

    Agrobacterium tumefaciens is known to transfer parts of its tumour-inducing plasmid, the T-DNA, to plants, yeasts and filamentous fungi. We have used this system to transform germinating basidiospores and vegetative mycelium of a commercial strain of the cultivated basidiomycete Agaricus bisporus. Analysis of transformants shows that the T-DNA integrates at random sites into the host genome and that the selection marker is stable during mitosis and meiosis. The Agrobacterium system allows the transformation of both homokaryons and heterokaryons of A. bisporus. Also, both karyotypes of an heterokaryon can be transformed simultaneously. Furthermore, this is the first report on the transformation of vegetative mycelium of a commercial strain of A. bisporus.
    Original languageEnglish
    Pages (from-to)35-39
    JournalCurrent Genetics
    Volume39
    Issue number1
    DOIs
    Publication statusPublished - 2001

    Keywords

    • mediated transformation
    • filamentous fungi
    • recombination
    • integration
    • genome

    Fingerprint Dive into the research topics of 'Transformation of the cultivated mushroom Agaricus bisporus (Lange) using T-DNA from Agrobacterium tumefaciens.'. Together they form a unique fingerprint.

    Cite this