Transformation methods for obtaining marker-free genetically modified plants

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14 Citations (Scopus)
Original languageEnglish
Title of host publicationPlant Transformation Technologies
EditorsC.N. Stewart, A. Touraev, V. Citovsky, T. Tzfira
PublisherBlackwell
Chapter15
Pages229-242
ISBN (Print)9780813821955
DOIs
Publication statusPublished - 2010

Keywords

  • Elimination of recombinase gene-cloning between recombination sites and selectable marker gene
  • Genetic modification, enriching existing cultivars-with new valuable characteristics
  • Marker elimination
  • Marker-free transformation methods-for generation of cisgenic or intragenic GM plants
  • Marker-free transformation technology
  • Selectable marker, prerequisite-for transgenic tissue or plant selection
  • Selectable markers and public concern-GM plants, selection systems leading to preferential growth of transformed cells
  • Sexual crosses, leading to loss of elite cultivar characteristics
  • Specific issues, associated-with transformation without selectable marker
  • Transformation methods, for obtaining marker-free genetically modified plants

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