Transfer of enteric pathogens to successive habitats as part of microbial cycles

A.M. Semenov, A.A. Kupriyanov, A.H.C. van Bruggen

Research output: Contribution to journalArticleAcademicpeer-review

45 Citations (Scopus)

Abstract

Escherichia coli O157:H7 gfp and Salmonella enterica Typhimurium gfp passed through six successive habitats within a microbial cycle. Pathogen cultures were introduced into cow dung or fodder. Microscopically observed cells and CFUs were monitored in fodder, dung, dung-soil mix, rhizosphere and phyllosphere of cress or oat plants grown in infested dung–soil mix, and in excrements of snails or mice fed with contaminated cress or oat shoots. Both methods were sensitive enough to monitor cells and CFUs throughout the chain. There was a positive correlation between cells and CFUs. Both pathogens declined through the successive habitats, but with unexpected increased densities on plants compared to dung–soil mix. Pathogen densities were higher in the phyllosphere than the rhizosphere of cress, but for oat plants this was reverse. Survival in dung was better after passage through the digestive tract of cows than after introduction of cultures into dung. Positive correlations between pathogens and copiotrophic bacteria (CB) and dissolved organic carbon (DOC) were observed in dung and dung-soil mixtures, but at low DOC contents CB densities were higher than pathogen densities. Thus, the pathogens are able to cycle through different habitats, surviving or growing better at high DOC concentrations, but maintaining population densities that are sufficiently high to cause disease in humans
Original languageEnglish
Pages (from-to)239-249
JournalMicrobial Ecology
Volume60
Issue number1
DOIs
Publication statusPublished - 2010

Fingerprint

pathogen
feces
pathogens
habitat
habitats
dissolved organic carbon
phyllosphere
oats
fodder
rhizosphere
forage
bacterium
Salmonella enterica
bacteria
cells
excreta
cattle manure
Escherichia coli O157
human diseases
digestive tract

Keywords

  • escherichia-coli o157-h7
  • manure-amended soil
  • salmonella-enterica
  • serovar typhimurium
  • indigenous microflora
  • foodborne illness
  • dairy farms
  • survival
  • contamination
  • lettuce

Cite this

Semenov, A.M. ; Kupriyanov, A.A. ; van Bruggen, A.H.C. / Transfer of enteric pathogens to successive habitats as part of microbial cycles. In: Microbial Ecology. 2010 ; Vol. 60, No. 1. pp. 239-249.
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abstract = "Escherichia coli O157:H7 gfp and Salmonella enterica Typhimurium gfp passed through six successive habitats within a microbial cycle. Pathogen cultures were introduced into cow dung or fodder. Microscopically observed cells and CFUs were monitored in fodder, dung, dung-soil mix, rhizosphere and phyllosphere of cress or oat plants grown in infested dung–soil mix, and in excrements of snails or mice fed with contaminated cress or oat shoots. Both methods were sensitive enough to monitor cells and CFUs throughout the chain. There was a positive correlation between cells and CFUs. Both pathogens declined through the successive habitats, but with unexpected increased densities on plants compared to dung–soil mix. Pathogen densities were higher in the phyllosphere than the rhizosphere of cress, but for oat plants this was reverse. Survival in dung was better after passage through the digestive tract of cows than after introduction of cultures into dung. Positive correlations between pathogens and copiotrophic bacteria (CB) and dissolved organic carbon (DOC) were observed in dung and dung-soil mixtures, but at low DOC contents CB densities were higher than pathogen densities. Thus, the pathogens are able to cycle through different habitats, surviving or growing better at high DOC concentrations, but maintaining population densities that are sufficiently high to cause disease in humans",
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Transfer of enteric pathogens to successive habitats as part of microbial cycles. / Semenov, A.M.; Kupriyanov, A.A.; van Bruggen, A.H.C.

In: Microbial Ecology, Vol. 60, No. 1, 2010, p. 239-249.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Transfer of enteric pathogens to successive habitats as part of microbial cycles

AU - Semenov, A.M.

AU - Kupriyanov, A.A.

AU - van Bruggen, A.H.C.

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AB - Escherichia coli O157:H7 gfp and Salmonella enterica Typhimurium gfp passed through six successive habitats within a microbial cycle. Pathogen cultures were introduced into cow dung or fodder. Microscopically observed cells and CFUs were monitored in fodder, dung, dung-soil mix, rhizosphere and phyllosphere of cress or oat plants grown in infested dung–soil mix, and in excrements of snails or mice fed with contaminated cress or oat shoots. Both methods were sensitive enough to monitor cells and CFUs throughout the chain. There was a positive correlation between cells and CFUs. Both pathogens declined through the successive habitats, but with unexpected increased densities on plants compared to dung–soil mix. Pathogen densities were higher in the phyllosphere than the rhizosphere of cress, but for oat plants this was reverse. Survival in dung was better after passage through the digestive tract of cows than after introduction of cultures into dung. Positive correlations between pathogens and copiotrophic bacteria (CB) and dissolved organic carbon (DOC) were observed in dung and dung-soil mixtures, but at low DOC contents CB densities were higher than pathogen densities. Thus, the pathogens are able to cycle through different habitats, surviving or growing better at high DOC concentrations, but maintaining population densities that are sufficiently high to cause disease in humans

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KW - foodborne illness

KW - dairy farms

KW - survival

KW - contamination

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