Transcriptome and proteome analyses of proteases in biotroph fungal pathogen Cladosporium fulvum

Mansoor Karimi Jashni*, Ate van der Burgt, Evy Battaglia, Rahim Mehrabi, Jérôme Collemare, Pierre J.G.M. de Wit

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Proteases are key components of the hydrolytic enzyme arsenal employed by fungal pathogens to invade their host plants. The recent advances in -omics era have facilitated identification of functional proteases involved in plant-fungus interactions. By comparison of the publically available sequences of fungal genomes we found that the number of protease genes present in the genome of Cladosporium fulvum, a biotrophic tomato pathogen, is comparable with that of hemibiotrophs. To identify host plant inducible protease genes and their products, we performed transcriptome and proteome analyses of C. fulvumin vitro and in planta by means of RNA-Seq/RT-qPCR and mass spectrometry. Transcriptome data showed that 14 out of the 59 predicted proteases are expressed during in vitro and in planta growth of C. fulvum, of which nine belong to serine proteases S8 and S10 and the rest belong to metallo- and aspartic proteases. Mass spectrometry confirmed the presence of six proteases at proteome level during plant infection. Expression of limited number of proteases by C. fulvum might sustain biotrophic growth and benefits its stealth pathogenesis.

Original languageEnglish
Pages (from-to)377-386
Number of pages10
JournalJournal of Plant Pathology
Volume102
Early online date5 Nov 2019
DOIs
Publication statusPublished - May 2020

Keywords

  • Cladosoprium fulvum
  • Fungal proteases
  • Gene expression
  • Plant-microbe interaction
  • Tomato pathogen

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