Transcriptome and proteome analyses of proteases in biotroph fungal pathogen Cladosporium fulvum

Mansoor Karimi Jashni*, Ate van der Burgt, Evy Battaglia, Rahim Mehrabi, Jérôme Collemare, Pierre J.G.M. de Wit

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Proteases are key components of the hydrolytic enzyme arsenal employed by fungal pathogens to invade their host plants. The recent advances in -omics era have facilitated identification of functional proteases involved in plant-fungus interactions. By comparison of the publically available sequences of fungal genomes we found that the number of protease genes present in the genome of Cladosporium fulvum, a biotrophic tomato pathogen, is comparable with that of hemibiotrophs. To identify host plant inducible protease genes and their products, we performed transcriptome and proteome analyses of C. fulvumin vitro and in planta by means of RNA-Seq/RT-qPCR and mass spectrometry. Transcriptome data showed that 14 out of the 59 predicted proteases are expressed during in vitro and in planta growth of C. fulvum, of which nine belong to serine proteases S8 and S10 and the rest belong to metallo- and aspartic proteases. Mass spectrometry confirmed the presence of six proteases at proteome level during plant infection. Expression of limited number of proteases by C. fulvum might sustain biotrophic growth and benefits its stealth pathogenesis.

Original languageEnglish
Number of pages10
JournalJournal of Plant Pathology
DOIs
Publication statusE-pub ahead of print - 5 Nov 2019

Fingerprint

Passalora fulva
proteome
transcriptome
proteinases
pathogens
Plantae
host plants
mass spectrometry
aspartic proteinases
genome
serine proteinases
genes
pathogenesis
tomatoes
RNA
fungi
enzymes

Keywords

  • Cladosoprium fulvum
  • Fungal proteases
  • Gene expression
  • Plant-microbe interaction
  • Tomato pathogen

Cite this

@article{2212e839e6a04e409f36683e58a4b116,
title = "Transcriptome and proteome analyses of proteases in biotroph fungal pathogen Cladosporium fulvum",
abstract = "Proteases are key components of the hydrolytic enzyme arsenal employed by fungal pathogens to invade their host plants. The recent advances in -omics era have facilitated identification of functional proteases involved in plant-fungus interactions. By comparison of the publically available sequences of fungal genomes we found that the number of protease genes present in the genome of Cladosporium fulvum, a biotrophic tomato pathogen, is comparable with that of hemibiotrophs. To identify host plant inducible protease genes and their products, we performed transcriptome and proteome analyses of C. fulvumin vitro and in planta by means of RNA-Seq/RT-qPCR and mass spectrometry. Transcriptome data showed that 14 out of the 59 predicted proteases are expressed during in vitro and in planta growth of C. fulvum, of which nine belong to serine proteases S8 and S10 and the rest belong to metallo- and aspartic proteases. Mass spectrometry confirmed the presence of six proteases at proteome level during plant infection. Expression of limited number of proteases by C. fulvum might sustain biotrophic growth and benefits its stealth pathogenesis.",
keywords = "Cladosoprium fulvum, Fungal proteases, Gene expression, Plant-microbe interaction, Tomato pathogen",
author = "{Karimi Jashni}, Mansoor and {van der Burgt}, Ate and Evy Battaglia and Rahim Mehrabi and J{\'e}r{\^o}me Collemare and {de Wit}, {Pierre J.G.M.}",
year = "2019",
month = "11",
day = "5",
doi = "10.1007/s42161-019-00433-0",
language = "English",
journal = "Journal of Plant Pathology: rivista di patologia vegetale",
issn = "1125-4653",
publisher = "Edizioni ETS Pisa",

}

Transcriptome and proteome analyses of proteases in biotroph fungal pathogen Cladosporium fulvum. / Karimi Jashni, Mansoor; van der Burgt, Ate; Battaglia, Evy; Mehrabi, Rahim; Collemare, Jérôme; de Wit, Pierre J.G.M.

In: Journal of Plant Pathology, 05.11.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Transcriptome and proteome analyses of proteases in biotroph fungal pathogen Cladosporium fulvum

AU - Karimi Jashni, Mansoor

AU - van der Burgt, Ate

AU - Battaglia, Evy

AU - Mehrabi, Rahim

AU - Collemare, Jérôme

AU - de Wit, Pierre J.G.M.

PY - 2019/11/5

Y1 - 2019/11/5

N2 - Proteases are key components of the hydrolytic enzyme arsenal employed by fungal pathogens to invade their host plants. The recent advances in -omics era have facilitated identification of functional proteases involved in plant-fungus interactions. By comparison of the publically available sequences of fungal genomes we found that the number of protease genes present in the genome of Cladosporium fulvum, a biotrophic tomato pathogen, is comparable with that of hemibiotrophs. To identify host plant inducible protease genes and their products, we performed transcriptome and proteome analyses of C. fulvumin vitro and in planta by means of RNA-Seq/RT-qPCR and mass spectrometry. Transcriptome data showed that 14 out of the 59 predicted proteases are expressed during in vitro and in planta growth of C. fulvum, of which nine belong to serine proteases S8 and S10 and the rest belong to metallo- and aspartic proteases. Mass spectrometry confirmed the presence of six proteases at proteome level during plant infection. Expression of limited number of proteases by C. fulvum might sustain biotrophic growth and benefits its stealth pathogenesis.

AB - Proteases are key components of the hydrolytic enzyme arsenal employed by fungal pathogens to invade their host plants. The recent advances in -omics era have facilitated identification of functional proteases involved in plant-fungus interactions. By comparison of the publically available sequences of fungal genomes we found that the number of protease genes present in the genome of Cladosporium fulvum, a biotrophic tomato pathogen, is comparable with that of hemibiotrophs. To identify host plant inducible protease genes and their products, we performed transcriptome and proteome analyses of C. fulvumin vitro and in planta by means of RNA-Seq/RT-qPCR and mass spectrometry. Transcriptome data showed that 14 out of the 59 predicted proteases are expressed during in vitro and in planta growth of C. fulvum, of which nine belong to serine proteases S8 and S10 and the rest belong to metallo- and aspartic proteases. Mass spectrometry confirmed the presence of six proteases at proteome level during plant infection. Expression of limited number of proteases by C. fulvum might sustain biotrophic growth and benefits its stealth pathogenesis.

KW - Cladosoprium fulvum

KW - Fungal proteases

KW - Gene expression

KW - Plant-microbe interaction

KW - Tomato pathogen

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M3 - Article

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SN - 1125-4653

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