Transcriptome and functional analysis of apothecium development in Botrytis cinerea

J.A.L. van Kan, R.B. Terhem, A. Rodenburg, J.H.M. Stassen

Research output: Chapter in Book/Report/Conference proceedingAbstract


Botrytis cinerea is a plant pathogenic ascomycete producing sexual fruiting bodies named apothecia. We performed RNA-seq analysis of sclerotia, three stages of apothecium development and ascospores. Transcriptomes were compared between successive developmental stages in order to describe transcriptional changes occurring during developmental transitions. Strikingly, more than 5000 genes were differentially expressed between mature apothecia and ascospores. Ascospores expressed several genes encoding virulence factors, even prior to interaction with host plants. Secondly, cluster analysis identified six sets of genes with common transcriptional profiles over the developmental stages. Interestingly, ~80% of the genes that were specifically expressed in mature apothecia were of unknown function and exclusively had homologs within the order Helotiales, but no homologs in fungi producing other types of ascocarps. This suggest that the apothecium is a structure that evolved independently from other fruiting bodies (cleistothecia, perithecia or pseudothecia).
Original languageEnglish
Title of host publicationBook of Abstracts 28th Fungal Genetics Conference
Publication statusPublished - 2015
Event28th Fungal Genetics Conference, Pacific Grove, CA, USA -
Duration: 17 Mar 201522 Mar 2015


Conference28th Fungal Genetics Conference, Pacific Grove, CA, USA

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