Transcriptional and metabolite analysis reveal a shift in direct and indirect defences in response to spider-mite infestation in cucumber (Cucumis sativus)

Jun He, Harro J. Bouwmeester, Marcel Dicke, Iris F. Kappers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Key message: Cucumber plants adapt their transcriptome and metabolome as result of spider mite infestation with opposite consequences for direct and indirect defences in two genotypes. Abstract: Plants respond to arthropod attack with the rearrangement of their transcriptome which lead to subsequent phenotypic changes in the plants’ metabolome. Here, we analysed transcriptomic and metabolite responses of two cucumber (Cucumis sativus) genotypes to chelicerate spider mites (Tetranychus urticae) during the first 3 days of infestation. Genes associated with the metabolism of jasmonates, phenylpropanoids, terpenoids and l-phenylalanine were most strongly upregulated. Also, genes involved in the biosynthesis of precursors for indirect defence-related terpenoids were upregulated while those involved in the biosynthesis of direct defence-related cucurbitacin C were downregulated. Consistent with the observed transcriptional changes, terpenoid emission increased and cucurbitacin C content decreased during early spider-mite herbivory. To further study the regulatory network that underlies induced defence to spider mites, differentially expressed genes that encode transcription factors (TFs) were analysed. Correlation analysis of the expression of TF genes with metabolism-associated genes resulted in putative identification of regulators of herbivore-induced terpenoid, green-leaf volatiles and cucurbitacin biosynthesis. Our data provide a global image of the transcriptional changes in cucumber leaves in response to spider-mite herbivory and that of metabolites that are potentially involved in the regulation of induced direct and indirect defences against spider-mite herbivory.

Original languageEnglish
Pages (from-to)489-505
JournalPlant Molecular Biology
Volume103
Early online date18 Apr 2020
DOIs
Publication statusPublished - Jul 2020

Keywords

  • Cucumis sativus
  • Herbivores
  • Secondary metabolism
  • Transcriptome
  • Two-spotted spider mites
  • Volatile organic compounds

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    Transcriptional and metabolite analysis reveal a shift in direct and indirect defences in response to spider‑mite infestation in cucumber (Cucumis sativus)

    He, J., Bouwmeester, H. J., Dicke, M. & Kappers, I. F., 29 Apr 2020, In : Plant Molecular Biology.

    Research output: Contribution to journalComment/Letter to the editorAcademic

    Open Access
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