Transcription factor mediated control of anthocyanin biosynthesis in vegetative tissues

N.S. Outchkourov, R.B. Karlova, Matthijs Hölscher, Xandra Schrama, I. Blilou, E.J. Jongedijk, C. Diez Simon, A.D.J. van Dijk, H.J. Bosch, R.D. Hall, M.J. Beekwilder

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Plants accumulate secondary metabolites to adapt to environmental conditions. These compounds, here exemplified by the purple-colored anthocyanins, are accumulated upon high temperatures, UV-light, drought, and nutrient deficiencies, and may contribute to tolerance to these stresses. Producing compounds is often part of a more broad response of the plant to changes in the environment. Here we investigate how a transcription-factor-mediated program for controlling anthocyanin biosynthesis also has effects on formation of specialized cell structures and changes in the plant root architecture. A systems biology approach was developed in tomato (Solanum lycopersicum) for coordinated induction of biosynthesis of anthocyanins, in a tissue- and development-independent manner. A transcription factor couple from Antirrhinum that is known to control anthocyanin biosynthesis was introduced in tomato under control of a dexamethasone-inducible promoter. By application of dexamethasone, anthocyanin formation was induced within 24 h in vegetative tissues and in undifferentiated cells. Profiles of metabolites and gene expression were analyzed in several tomato tissues. Changes in concentration of anthocyanins and other phenolic compounds were observed in all tested tissues, accompanied by induction of the biosynthetic pathways leading from Glc to anthocyanins. A number of pathways that are not known to be involved in anthocyanin biosynthesis were observed to be regulated. Anthocyanin-producing plants displayed profound physiological and architectural changes, depending on the tissue, including root branching, root epithelial cell morphology, seed germination, and leaf conductance. The inducible anthocyanin-production system reveals a range of phenomena that accompanies anthocyanin biosynthesis in tomato, including adaptions of the plants architecture and physiology.
LanguageEnglish
Pages1862-1878
JournalPlant Physiology
Volume176
Issue number2
Early online date6 Feb 2018
DOIs
Publication statusPublished - Feb 2018

Fingerprint

Anthocyanins
anthocyanins
Transcription Factors
transcription factors
biosynthesis
Lycopersicon esculentum
tomatoes
dexamethasone
Dexamethasone
tissues
Antirrhinum
Plant Physiological Phenomena
leaf conductance
Plant Roots
Systems Biology
Cotyledon
Droughts
cell structures
Biosynthetic Pathways
plant physiology

Cite this

Outchkourov, N.S. ; Karlova, R.B. ; Hölscher, Matthijs ; Schrama, Xandra ; Blilou, I. ; Jongedijk, E.J. ; Diez Simon, C. ; van Dijk, A.D.J. ; Bosch, H.J. ; Hall, R.D. ; Beekwilder, M.J. / Transcription factor mediated control of anthocyanin biosynthesis in vegetative tissues. In: Plant Physiology. 2018 ; Vol. 176, No. 2. pp. 1862-1878.
@article{2e07b80249014376b435971ef604bb13,
title = "Transcription factor mediated control of anthocyanin biosynthesis in vegetative tissues",
abstract = "Plants accumulate secondary metabolites to adapt to environmental conditions. These compounds, here exemplified by the purple-colored anthocyanins, are accumulated upon high temperatures, UV-light, drought, and nutrient deficiencies, and may contribute to tolerance to these stresses. Producing compounds is often part of a more broad response of the plant to changes in the environment. Here we investigate how a transcription-factor-mediated program for controlling anthocyanin biosynthesis also has effects on formation of specialized cell structures and changes in the plant root architecture. A systems biology approach was developed in tomato (Solanum lycopersicum) for coordinated induction of biosynthesis of anthocyanins, in a tissue- and development-independent manner. A transcription factor couple from Antirrhinum that is known to control anthocyanin biosynthesis was introduced in tomato under control of a dexamethasone-inducible promoter. By application of dexamethasone, anthocyanin formation was induced within 24 h in vegetative tissues and in undifferentiated cells. Profiles of metabolites and gene expression were analyzed in several tomato tissues. Changes in concentration of anthocyanins and other phenolic compounds were observed in all tested tissues, accompanied by induction of the biosynthetic pathways leading from Glc to anthocyanins. A number of pathways that are not known to be involved in anthocyanin biosynthesis were observed to be regulated. Anthocyanin-producing plants displayed profound physiological and architectural changes, depending on the tissue, including root branching, root epithelial cell morphology, seed germination, and leaf conductance. The inducible anthocyanin-production system reveals a range of phenomena that accompanies anthocyanin biosynthesis in tomato, including adaptions of the plants architecture and physiology.",
author = "N.S. Outchkourov and R.B. Karlova and Matthijs H{\"o}lscher and Xandra Schrama and I. Blilou and E.J. Jongedijk and {Diez Simon}, C. and {van Dijk}, A.D.J. and H.J. Bosch and R.D. Hall and M.J. Beekwilder",
year = "2018",
month = "2",
doi = "10.1104/pp.17.01662",
language = "English",
volume = "176",
pages = "1862--1878",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "2",

}

Transcription factor mediated control of anthocyanin biosynthesis in vegetative tissues. / Outchkourov, N.S.; Karlova, R.B.; Hölscher, Matthijs; Schrama, Xandra; Blilou, I.; Jongedijk, E.J.; Diez Simon, C.; van Dijk, A.D.J.; Bosch, H.J.; Hall, R.D.; Beekwilder, M.J.

In: Plant Physiology, Vol. 176, No. 2, 02.2018, p. 1862-1878.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Transcription factor mediated control of anthocyanin biosynthesis in vegetative tissues

AU - Outchkourov, N.S.

AU - Karlova, R.B.

AU - Hölscher, Matthijs

AU - Schrama, Xandra

AU - Blilou, I.

AU - Jongedijk, E.J.

AU - Diez Simon, C.

AU - van Dijk, A.D.J.

AU - Bosch, H.J.

AU - Hall, R.D.

AU - Beekwilder, M.J.

PY - 2018/2

Y1 - 2018/2

N2 - Plants accumulate secondary metabolites to adapt to environmental conditions. These compounds, here exemplified by the purple-colored anthocyanins, are accumulated upon high temperatures, UV-light, drought, and nutrient deficiencies, and may contribute to tolerance to these stresses. Producing compounds is often part of a more broad response of the plant to changes in the environment. Here we investigate how a transcription-factor-mediated program for controlling anthocyanin biosynthesis also has effects on formation of specialized cell structures and changes in the plant root architecture. A systems biology approach was developed in tomato (Solanum lycopersicum) for coordinated induction of biosynthesis of anthocyanins, in a tissue- and development-independent manner. A transcription factor couple from Antirrhinum that is known to control anthocyanin biosynthesis was introduced in tomato under control of a dexamethasone-inducible promoter. By application of dexamethasone, anthocyanin formation was induced within 24 h in vegetative tissues and in undifferentiated cells. Profiles of metabolites and gene expression were analyzed in several tomato tissues. Changes in concentration of anthocyanins and other phenolic compounds were observed in all tested tissues, accompanied by induction of the biosynthetic pathways leading from Glc to anthocyanins. A number of pathways that are not known to be involved in anthocyanin biosynthesis were observed to be regulated. Anthocyanin-producing plants displayed profound physiological and architectural changes, depending on the tissue, including root branching, root epithelial cell morphology, seed germination, and leaf conductance. The inducible anthocyanin-production system reveals a range of phenomena that accompanies anthocyanin biosynthesis in tomato, including adaptions of the plants architecture and physiology.

AB - Plants accumulate secondary metabolites to adapt to environmental conditions. These compounds, here exemplified by the purple-colored anthocyanins, are accumulated upon high temperatures, UV-light, drought, and nutrient deficiencies, and may contribute to tolerance to these stresses. Producing compounds is often part of a more broad response of the plant to changes in the environment. Here we investigate how a transcription-factor-mediated program for controlling anthocyanin biosynthesis also has effects on formation of specialized cell structures and changes in the plant root architecture. A systems biology approach was developed in tomato (Solanum lycopersicum) for coordinated induction of biosynthesis of anthocyanins, in a tissue- and development-independent manner. A transcription factor couple from Antirrhinum that is known to control anthocyanin biosynthesis was introduced in tomato under control of a dexamethasone-inducible promoter. By application of dexamethasone, anthocyanin formation was induced within 24 h in vegetative tissues and in undifferentiated cells. Profiles of metabolites and gene expression were analyzed in several tomato tissues. Changes in concentration of anthocyanins and other phenolic compounds were observed in all tested tissues, accompanied by induction of the biosynthetic pathways leading from Glc to anthocyanins. A number of pathways that are not known to be involved in anthocyanin biosynthesis were observed to be regulated. Anthocyanin-producing plants displayed profound physiological and architectural changes, depending on the tissue, including root branching, root epithelial cell morphology, seed germination, and leaf conductance. The inducible anthocyanin-production system reveals a range of phenomena that accompanies anthocyanin biosynthesis in tomato, including adaptions of the plants architecture and physiology.

U2 - 10.1104/pp.17.01662

DO - 10.1104/pp.17.01662

M3 - Article

VL - 176

SP - 1862

EP - 1878

JO - Plant Physiology

T2 - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 2

ER -

Outchkourov NS, Karlova RB, Hölscher M, Schrama X, Blilou I, Jongedijk EJ et al. Transcription factor mediated control of anthocyanin biosynthesis in vegetative tissues. Plant Physiology. 2018 Feb;176(2):1862-1878. https://doi.org/10.1104/pp.17.01662