Transcription analysis of apple fruit development using cDNA microarrays

V. Soglio, F. Costa, J.W. Molthoff, M. Weemen-Hendriks, H.J. Schouten, L. Gianfranceschi

    Research output: Contribution to journalArticleAcademicpeer-review

    28 Citations (Scopus)

    Abstract

    The knowledge of the molecular mechanisms underlying fruit quality traits is fundamental to devise efficient marker-assisted selection strategies and to improve apple breeding. In this study, cDNA microarray technology was used to identify genes whose expression changes during fruit development and maturation thus potentially involved in fruit quality traits. The expression profile of 1,536 transcripts was analysed by microarray hybridisation. A total of 177 genes resulted to be differentially expressed in at least one of the developmental stages considered. Gene ontology annotation was employed to univocally describe gene function, while cluster analysis allowed grouping genes according to their expression profile. An overview of the transcriptional changes and of the metabolic pathways involved in fruit development was obtained. As expected, August and September are the two months where the largest number of differentially expressed genes was observed. In particular, 85 genes resulted to be up-regulated in September. Even though most of the differentially expressed genes are involved in primary metabolism, several other interesting functions were detected and will be presented.
    Original languageEnglish
    Pages (from-to)685-698
    JournalTree Genetics and Genomes
    Volume5
    Issue number4
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Malus
    Oligonucleotide Array Sequence Analysis
    fruiting
    Fruit
    transcription (genetics)
    fruit
    apples
    gene
    Genes
    genes
    microarray technology
    fruit quality
    Molecular Sequence Annotation
    Gene Ontology
    Metabolic Networks and Pathways
    Breeding
    Cluster Analysis
    analysis
    developmental stage
    marker-assisted selection

    Keywords

    • expressed-sequence-tags
    • beta-cyanoalanine synthase
    • malus-pumila mill.
    • gene-expression
    • ethylene biosynthesis
    • domestica borkh.
    • tomato
    • model
    • identification
    • maturation

    Cite this

    @article{e50171b20df64b30afdc7b29a78e5694,
    title = "Transcription analysis of apple fruit development using cDNA microarrays",
    abstract = "The knowledge of the molecular mechanisms underlying fruit quality traits is fundamental to devise efficient marker-assisted selection strategies and to improve apple breeding. In this study, cDNA microarray technology was used to identify genes whose expression changes during fruit development and maturation thus potentially involved in fruit quality traits. The expression profile of 1,536 transcripts was analysed by microarray hybridisation. A total of 177 genes resulted to be differentially expressed in at least one of the developmental stages considered. Gene ontology annotation was employed to univocally describe gene function, while cluster analysis allowed grouping genes according to their expression profile. An overview of the transcriptional changes and of the metabolic pathways involved in fruit development was obtained. As expected, August and September are the two months where the largest number of differentially expressed genes was observed. In particular, 85 genes resulted to be up-regulated in September. Even though most of the differentially expressed genes are involved in primary metabolism, several other interesting functions were detected and will be presented.",
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    author = "V. Soglio and F. Costa and J.W. Molthoff and M. Weemen-Hendriks and H.J. Schouten and L. Gianfranceschi",
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    Transcription analysis of apple fruit development using cDNA microarrays. / Soglio, V.; Costa, F.; Molthoff, J.W.; Weemen-Hendriks, M.; Schouten, H.J.; Gianfranceschi, L.

    In: Tree Genetics and Genomes, Vol. 5, No. 4, 2009, p. 685-698.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Soglio, V.

    AU - Costa, F.

    AU - Molthoff, J.W.

    AU - Weemen-Hendriks, M.

    AU - Schouten, H.J.

    AU - Gianfranceschi, L.

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    N2 - The knowledge of the molecular mechanisms underlying fruit quality traits is fundamental to devise efficient marker-assisted selection strategies and to improve apple breeding. In this study, cDNA microarray technology was used to identify genes whose expression changes during fruit development and maturation thus potentially involved in fruit quality traits. The expression profile of 1,536 transcripts was analysed by microarray hybridisation. A total of 177 genes resulted to be differentially expressed in at least one of the developmental stages considered. Gene ontology annotation was employed to univocally describe gene function, while cluster analysis allowed grouping genes according to their expression profile. An overview of the transcriptional changes and of the metabolic pathways involved in fruit development was obtained. As expected, August and September are the two months where the largest number of differentially expressed genes was observed. In particular, 85 genes resulted to be up-regulated in September. Even though most of the differentially expressed genes are involved in primary metabolism, several other interesting functions were detected and will be presented.

    AB - The knowledge of the molecular mechanisms underlying fruit quality traits is fundamental to devise efficient marker-assisted selection strategies and to improve apple breeding. In this study, cDNA microarray technology was used to identify genes whose expression changes during fruit development and maturation thus potentially involved in fruit quality traits. The expression profile of 1,536 transcripts was analysed by microarray hybridisation. A total of 177 genes resulted to be differentially expressed in at least one of the developmental stages considered. Gene ontology annotation was employed to univocally describe gene function, while cluster analysis allowed grouping genes according to their expression profile. An overview of the transcriptional changes and of the metabolic pathways involved in fruit development was obtained. As expected, August and September are the two months where the largest number of differentially expressed genes was observed. In particular, 85 genes resulted to be up-regulated in September. Even though most of the differentially expressed genes are involved in primary metabolism, several other interesting functions were detected and will be presented.

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    KW - malus-pumila mill.

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    KW - ethylene biosynthesis

    KW - domestica borkh.

    KW - tomato

    KW - model

    KW - identification

    KW - maturation

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