Tracking the Body, Wing, and Leg Kinematics of Moving Mosquitoes

Hedrick L. Tyson*, Andrew K. Dickerson, F.T. Muijres, R.P.M. Pieters

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)


In this protocol, we discuss general techniques for tracking the three-dimensional (3D) locations of the mosquito body, wings, legs, or other features of interest using videos. Tracking data must be acquired to produce detailed kinematics of moving mosquitoes. The software of focus for this protocol, DLTdv, was chosen for its widespread use and excellent support and because it is open-source. In addition, DLTdv allows both manual and automatic tracking. The automatic tracking can be done using a classic machine vision or machine-learning algorithm. The software supports both single-camera analysis and multicamera systems and can take advantage of sophisticated calibration algorithms, both for intrinsic lens distortion correction and for 3D DLT-based reconstruction. For this protocol, we assume all kinematic data is acquired post hoc through video analysis.
Translated title of the contributionHet volgen van de lichaams-, vleugel- en pootkinematica van bewegende muggen
Original languageEnglish
Article numberpdb.prot107928
JournalCold Spring Harbor Protocols
Issue number2
Early online date28 Sept 2022
Publication statusPublished - Feb 2023


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