Tracking Functional Guilds: Dehalococcoides spp. in European River Basins Contaminated with Hexachlorobenzene

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Abstract

Hexachlorobenzene (HCB) has been widely used in chemical manufacturing processes and as pesticide. Due to its resistance to biological degradation, HCB mainly accumulated in fresh water bodies and agricultural soils. Dehalococcoides spp., anaerobic dechlorinating bacteria that are capable of degrading HCB, were previously isolated from river sediments. Yet there is limited knowledge about the abundance, diversity and activity of this genus in the environment. This study focused on the molecular analysis of the composition and abundance of active Dehalococcoides spp. in HCB-contaminated European river basins. 16S ribosomal RNA-based real-time quantitative PCR and denaturing gradient gel electrophoresis in combination with multivariate statistics were applied. Moreover, a functional gene array was used to determine reductive dehalogenase (rdh) gene diversity. Spatial and temporal fluctuations were observed not only in the abundance of Dehalococcoides spp. but also in the composition of the populations and rdh gene diversity. Multivariate statistics revealed that Dehalococcoides spp. abundance is primarily affected by spatial differences, whereas species composition is under the influence of several environmental parameters, such as seasonal changes, total organic carbon and/or nitrogen content and HCB contamination. This study provides new insight in the natural occurrence and dynamics of active Dehalococcoides spp. in HCB contaminated river basins
Original languageEnglish
Pages (from-to)4696-4704
JournalApplied and Environmental Microbiology
Volume75
Issue number14
DOIs
Publication statusPublished - 2009

Keywords

  • 16s ribosomal-rna
  • lower ebro river
  • enrichment culture
  • environmental processes
  • microbial-communities
  • chlorinated benzenes
  • ethenogenes 195
  • vinyl-chloride
  • sequence data
  • ne spain

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