TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance

Jie Wang, Qihang Jiang, Roman Pleskot, Peter Grones, Elmehdi Bahafid, Grégoire Denay, Carlos Galván-Ampudia, Xiangyu Xu, Michael Vandorpe, Evelien Mylle, Ive De Smet, Teva Vernoux, Rüdiger Simon, Moritz K. Nowack, Daniel Van Damme*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Endocytosis regulates the turnover of cell surface localized receptors, which are crucial for plants to rapidly respond to stimuli. The evolutionary ancient TPLATE complex (TPC) plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of single TPC subunits causes male sterility and seedling lethality phenotypes, complicating analysis of the roles of TPC during plant development. Partially functional alleles of TPC subunits however only cause mild developmental deviations. Here, we took advantage of the partially functional TPLATE allele, WDXM2, to investigate a role for TPC-dependent endocytosis in receptor-mediated signaling. We discovered that reduced TPC-dependent endocytosis confers a hypersensitivity to very low doses of CLAVATA3 peptide signaling. This hypersensitivity correlated with the abundance of the CLAVATA3 receptor protein kinase CLAVATA1 at the plasma membrane. Genetic and biochemical analysis as well as live-cell imaging revealed that TPC-dependent regulation of CLAVATA3-dependent internalization of CLAVATA1 from the plasma membrane is required for shoot stem cell homeostasis. Our findings provide evidence that TPC-mediated endocytosis and degradation of CLAVATA1 is a mechanism to dampen CLAVATA3-mediated signaling during plant development.

Original languageEnglish
Article numbere54709
JournalEmbo Reports
Volume24
Issue number9
DOIs
Publication statusPublished - 6 Sept 2023
Externally publishedYes

Keywords

  • clathrin-mediated endocytosis
  • CLAVATA3-CLAVATA1 signaling
  • shoot apical meristem maintenance
  • TPLATE complex

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