TY - JOUR
T1 - Toxin- and cadmium-induced cell death events in tomato suspension cells resemble features of hypersensitive response
AU - Iakimova, E.T.
AU - Woltering, E.J.
AU - Yordanova, Z.P.
PY - 2007
Y1 - 2007
N2 - Elicitors of different origin (fumonisin B1, fungal toxin), camptothecin (alkaloid
from Camptotheca acuminata), mastoparan (wasp venom) and the heavy metal
(cadmium) were tested for their ability to induce programmed cell death (PCD) in
a model system of tomato cell culture, line MsK8. By employing a pharmacological
approach the involvement of proteolysis, oxidative stress and ethylene in the suicidal
cascade is shown. The caspase-specific peptide inhibitors: irreversible caspase-1
(ICE)-inhibitor acyl-Tyr-Val-Ala-Asp-chloromethylketone and the broad range caspase
inhibitor benzyoxycarbonyl-Asp-2,6-dichlorobenzoyloxymethylketone effectively reduced
cell lethality providing a sound indication that in tomato suspension cells the applied
inducers promote cell death that resembles features typical for PCD. A lack of
inhibition occurred at mastoparan-induced cell death in response to the caspase
inhibitors, while the serine protease inhibitor aminoethylbenzenesulphonyl fluoride
caused substantial reduction of cell mortality. Significant inhibition was detected after
administration of ethylene inhibitor, aminoethoxyvinyl glicine and the antioxidants Lgalactonic
acid -¿-galactone and catalase. The results indicate that the cell death
response at the exposure to biotic and abiotic stressors may employ an activation of
similar cell death pathways and that caspase-like- and non-caspase-like-dependent
biochemical processes may be operative. In addition, the presented comparative study
suggests that the reaction of tomato suspension cells to diverse cell death stimulating
compounds at least partially coincides with the cell death machinery involved in the
plant hypersensitive response and during PCD in animal cells.
AB - Elicitors of different origin (fumonisin B1, fungal toxin), camptothecin (alkaloid
from Camptotheca acuminata), mastoparan (wasp venom) and the heavy metal
(cadmium) were tested for their ability to induce programmed cell death (PCD) in
a model system of tomato cell culture, line MsK8. By employing a pharmacological
approach the involvement of proteolysis, oxidative stress and ethylene in the suicidal
cascade is shown. The caspase-specific peptide inhibitors: irreversible caspase-1
(ICE)-inhibitor acyl-Tyr-Val-Ala-Asp-chloromethylketone and the broad range caspase
inhibitor benzyoxycarbonyl-Asp-2,6-dichlorobenzoyloxymethylketone effectively reduced
cell lethality providing a sound indication that in tomato suspension cells the applied
inducers promote cell death that resembles features typical for PCD. A lack of
inhibition occurred at mastoparan-induced cell death in response to the caspase
inhibitors, while the serine protease inhibitor aminoethylbenzenesulphonyl fluoride
caused substantial reduction of cell mortality. Significant inhibition was detected after
administration of ethylene inhibitor, aminoethoxyvinyl glicine and the antioxidants Lgalactonic
acid -¿-galactone and catalase. The results indicate that the cell death
response at the exposure to biotic and abiotic stressors may employ an activation of
similar cell death pathways and that caspase-like- and non-caspase-like-dependent
biochemical processes may be operative. In addition, the presented comparative study
suggests that the reaction of tomato suspension cells to diverse cell death stimulating
compounds at least partially coincides with the cell death machinery involved in the
plant hypersensitive response and during PCD in animal cells.
M3 - Article
VL - 15
SP - 15
EP - 19
JO - Journal of Fruit and Ornamental Plant Research
JF - Journal of Fruit and Ornamental Plant Research
SN - 1231-0948
ER -