Toxicokinetics and bioaccumulation of silver sulfide nanoparticles in benthic invertebrates in an indoor stream mesocosm

Patrícia V. Silva*, Ana Rita R. Silva, Nathaniel J. Clark, Joanne Vassallo, Marta Baccaro, Neja Medvešček, Magdalena Grgić, Abel Ferreira, Martí Busquets-Fité, Kerstin Jurkschat, Anastasios G. Papadiamantis, Victor Puntes, Iseult Lynch, Claus Svendsen, Nico W. van den Brink, Richard D. Handy, Cornelis A.M. van Gestel, Susana Loureiro

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Mesocosms allow the simulation of environmentally relevant conditions and can be used to establish more realistic scenarios of organism exposure to nanoparticles. An indoor mesocosm experiment simulating an aquatic stream ecosystem was conducted to assess the toxicokinetics and bioaccumulation of silver sulfide nanoparticles (Ag2S NPs) and AgNO3 in the freshwater invertebrates Girardia tigrina, Physa acuta and Chironomus riparius, and determine if previous single-species tests can predict bioaccumulation in the mesocosm. Water was daily spiked at 10 μg Ag L−1. Ag concentrations in water and sediment reached values of 13.4 μg Ag L−1 and 0.30 μg Ag g−1 in the Ag2S NP exposure, and 12.8 μg Ag L−1 and 0.20 μg Ag g−1 in the AgNO3. Silver was bioaccumulated by the species from both treatments, but with approximately 1.5, 3 and 11 times higher body Ag concentrations in AgNO3 compared to Ag2S NP exposures in snails, chironomids and planarians, respectively. In the Ag2S NP exposures, the observed uptake was probably of the particulate form. This demonstrates that this more environmentally relevant Ag nanoform may be bioavailable for uptake by benthic organisms. Interspecies interactions likely occurred, namely predation (planarians fed on chironomids and snails), which somehow influenced Ag uptake/bioaccumulation, possibly by altering organisms´ foraging behaviour. Higher Ag uptake rate constants were determined for AgNO3 (0.64, 80.4 and 1.12 Lwater g−1organism day−1) than for Ag2S NPs (0.05, 2.65 and 0.32 Lwater g−1organism day−1) for planarians, snails and chironomids, respectively. Biomagnification under environmentally realistic exposure seemed to be low, although it was likely to occur in the food chain P. acuta to G. tigrina exposed to AgNO3. Single-species tests generally could not reliably predict Ag bioaccumulation in the more complex mesocosm scenario. This study provides methodologies/data to better understand exposure, toxicokinetics and bioaccumulation of Ag in complex systems, reinforcing the need to use mesocosm studies to improve the risk assessment of environmental contaminants, specifically NPs, in aquatic environments.

Original languageEnglish
Article number162160
Number of pages14
JournalScience of the Total Environment
Volume873
DOIs
Publication statusPublished - 15 May 2023

Keywords

  • Exposure routes
  • Nanomaterials
  • Risk assessment
  • Sediments
  • Single-species tests
  • Uptake and elimination

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