Toxicity of long chain fatty acids towards acetate conversion by Methanosaeta concilii and Methanosarcina mazei

Sérgio A. Silva, Andreia F. Salvador, Ana J. Cavaleiro*, M.A. Pereira, Fons Stams, M.M. Alves, Diana Z. Sousa

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

Long-chain fatty acids (LCFA) can inhibit methane production by methanogenic archaea. The effect of oleate and palmitate on pure cultures of Methanosaeta concilii and Methanosarcina mazei was assessed by comparing methane 11% and 64% methanogenic inhibition respectively. This study indicates that M. production rates from acetate before and after LCFA addition. For both methanogens, a sharp decrease in methane production (> 50%) was observed at 0.5 mmol L-1 oleate, and no methane was formed at concentrations higher than 2 mmol L-1 oleate. Palmitate was less inhibitory than oleate, and M. concilii was more tolerant to palmitate than M. mazei, with 2 mmol L-1 palmitate causing concilii and M. mazei tolerate LCFA concentrations similar to those previously described for hydrogenotrophic methanogens. In particular, the robustness of M. concilii might contribute to the observed prevalence of Methanosaeta species in anaerobic bioreactors used to treat LCFA-rich wastewater.

Original languageEnglish
Pages (from-to)514-518
JournalMicrobial Biotechnology
Volume9
Issue number4
DOIs
Publication statusPublished - 2016

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