Towards the production of genetically modified strawberries which are acceptable to consumers

J.G. Schaart, T.D. Kjellsen, R. Heggem, T.H. Iversen, H.J. Schouten, F.A. Krens

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

This manuscript discusses different aspects that are relevant to genetically modified strawberry plants with improved characteristics and ‘acceptable’ to consumers and growers of strawberry. It starts with a consumer acceptance survey, held in Norway, Denmark and the UK, studying public perception of genetic modification in general and specifically of genetically modified strawberries with altered properties. This study revealed that genetically modified plants are better accepted by consumers if only genes from the species itself are used for the genetic modification. Subsequently, the results of a functional analysis of the strawberry polygalacturonase inhibiting protein gene (FaPGIP) are described. This indicates that this gene is a possible candidate to induce resistance to Botrytis cinerea when upregulated in strawberry fruits. For this analysis, the FaPGIP gene was overexpressed in transgenic strawberry plants using the cauliflower mosaic virus 35S (CaMV35S) promoter. This showed that FaPGIP overexpression led to resistance to Botrytis in transgenic leaves. For the generation of intragenic (i.e. genetically modification using native genetic elements only) strawberry plants, a transformation vector was constructed in which FaPGIP was combined with a strawberry fruit-specific promoter and terminator that were isolated from a strawberry expansin gene (FaExp2). This vector also included elements that allow the elimination of (foreign) selectable marker genes after genetically modified plant lines have been established. Using this vector, genetically modified strawberry plants were produced that contained only genes from the species itself, and therefore these plants were called intragenic, rather than transgenic. Unfortunately, further evaluations of the intragenic strawberry plants could not demonstrate any enhanced level of resistance to Botrytis in fruits.
Original languageEnglish
Pages (from-to)102-107
JournalGenes, Genomes and Genomics
Volume5
Issue numberSp.issue 1
Publication statusPublished - 2011

Fingerprint

strawberries
polygalacturonase
genes
Botrytis
genetically modified organisms
genetic engineering
fruits
proteins
promoter regions
expansins
gene overexpression
consumer acceptance
Cauliflower mosaic virus
Botrytis cinerea
Denmark
Norway
growers

Cite this

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abstract = "This manuscript discusses different aspects that are relevant to genetically modified strawberry plants with improved characteristics and ‘acceptable’ to consumers and growers of strawberry. It starts with a consumer acceptance survey, held in Norway, Denmark and the UK, studying public perception of genetic modification in general and specifically of genetically modified strawberries with altered properties. This study revealed that genetically modified plants are better accepted by consumers if only genes from the species itself are used for the genetic modification. Subsequently, the results of a functional analysis of the strawberry polygalacturonase inhibiting protein gene (FaPGIP) are described. This indicates that this gene is a possible candidate to induce resistance to Botrytis cinerea when upregulated in strawberry fruits. For this analysis, the FaPGIP gene was overexpressed in transgenic strawberry plants using the cauliflower mosaic virus 35S (CaMV35S) promoter. This showed that FaPGIP overexpression led to resistance to Botrytis in transgenic leaves. For the generation of intragenic (i.e. genetically modification using native genetic elements only) strawberry plants, a transformation vector was constructed in which FaPGIP was combined with a strawberry fruit-specific promoter and terminator that were isolated from a strawberry expansin gene (FaExp2). This vector also included elements that allow the elimination of (foreign) selectable marker genes after genetically modified plant lines have been established. Using this vector, genetically modified strawberry plants were produced that contained only genes from the species itself, and therefore these plants were called intragenic, rather than transgenic. Unfortunately, further evaluations of the intragenic strawberry plants could not demonstrate any enhanced level of resistance to Botrytis in fruits.",
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Towards the production of genetically modified strawberries which are acceptable to consumers. / Schaart, J.G.; Kjellsen, T.D.; Heggem, R.; Iversen, T.H.; Schouten, H.J.; Krens, F.A.

In: Genes, Genomes and Genomics, Vol. 5, No. Sp.issue 1, 2011, p. 102-107.

Research output: Contribution to journalArticleAcademicpeer-review

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