Towards sorting of biolibraries using single-molecule fluorescence detection techniques

A.J.W.G. Visser, B.H. Kunst, H.J.H.G. Keller, A. Schots

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation or biolibraries, single-molecule detection techniques and rnicrofluidic devices. A high-throughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.
Original languageEnglish
Pages (from-to)173-179
JournalCurrent Pharmaceutical Biotechnology
Volume5
DOIs
Publication statusPublished - 2004

Keywords

  • cross-correlation spectroscopy
  • total analysis systems
  • phage-display
  • cell sorter
  • flow
  • biotechnology
  • technology
  • particles
  • channels
  • cloning

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