Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger

E.B.J. ten Buren, M.A.P. Karrenbelt, M. Lingemann, S. Chordia, Y. Deng, J.J. Hu, J.M. Verest, V. Wu, T.D.G. Bello Gonzalez, R.G.A. van Heck, D.I. Odoni, T. Schonewille, L. van der Straat, L.H. de Graaff, M.W.J. van Passel

Research output: Contribution to journalEditorialAcademicpeer-review

3 Citations (Scopus)

Abstract

Aspergillus niger is a filamentous fungus that is extensively used in industrial fermentations for protein expression and the production of organic acids. Inherent biosynthetic capabilities, such as the capacity to secrete these biomolecules in high amounts, make A. niger an attractive production host. Although A. niger is renowned for this ability, the knowledge of the molecular components that underlie its production capacity, intercellular trafficking processes and secretion mechanisms is far from complete. Here, we introduce a standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein. Expression of the GFP-actin construct facilitates visualization of the actin filaments of the cytoskeleton, whereas expression of the chromoprotein construct results in a clearly distinguishable red phenotype. These experimentally validated constructs constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena.
LanguageEnglish
Pages995-998
JournalACS synthetic biology
Volume3
Issue number12
DOIs
Publication statusPublished - 2014

Fingerprint

Aspergillus niger
Aspergillus
Cellular Structures
Organelles
Actins
Visualization
Actin Cytoskeleton
Organic acids
Biomolecules
Biomarkers
Fungi
Fermentation
Secretory Pathway
Fusion reactions
Codon
Proteins
Phenotype
Acids

Keywords

  • gene
  • hyphae

Cite this

ten Buren, E. B. J., Karrenbelt, M. A. P., Lingemann, M., Chordia, S., Deng, Y., Hu, J. J., ... van Passel, M. W. J. (2014). Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger. ACS synthetic biology, 3(12), 995-998. https://doi.org/10.1021/sb500304m
ten Buren, E.B.J. ; Karrenbelt, M.A.P. ; Lingemann, M. ; Chordia, S. ; Deng, Y. ; Hu, J.J. ; Verest, J.M. ; Wu, V. ; Bello Gonzalez, T.D.G. ; van Heck, R.G.A. ; Odoni, D.I. ; Schonewille, T. ; van der Straat, L. ; de Graaff, L.H. ; van Passel, M.W.J. / Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger. In: ACS synthetic biology. 2014 ; Vol. 3, No. 12. pp. 995-998.
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abstract = "Aspergillus niger is a filamentous fungus that is extensively used in industrial fermentations for protein expression and the production of organic acids. Inherent biosynthetic capabilities, such as the capacity to secrete these biomolecules in high amounts, make A. niger an attractive production host. Although A. niger is renowned for this ability, the knowledge of the molecular components that underlie its production capacity, intercellular trafficking processes and secretion mechanisms is far from complete. Here, we introduce a standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein. Expression of the GFP-actin construct facilitates visualization of the actin filaments of the cytoskeleton, whereas expression of the chromoprotein construct results in a clearly distinguishable red phenotype. These experimentally validated constructs constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena.",
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ten Buren, EBJ, Karrenbelt, MAP, Lingemann, M, Chordia, S, Deng, Y, Hu, JJ, Verest, JM, Wu, V, Bello Gonzalez, TDG, van Heck, RGA, Odoni, DI, Schonewille, T, van der Straat, L, de Graaff, LH & van Passel, MWJ 2014, 'Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger', ACS synthetic biology, vol. 3, no. 12, pp. 995-998. https://doi.org/10.1021/sb500304m

Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger. / ten Buren, E.B.J.; Karrenbelt, M.A.P.; Lingemann, M.; Chordia, S.; Deng, Y.; Hu, J.J.; Verest, J.M.; Wu, V.; Bello Gonzalez, T.D.G.; van Heck, R.G.A.; Odoni, D.I.; Schonewille, T.; van der Straat, L.; de Graaff, L.H.; van Passel, M.W.J.

In: ACS synthetic biology, Vol. 3, No. 12, 2014, p. 995-998.

Research output: Contribution to journalEditorialAcademicpeer-review

TY - JOUR

T1 - Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger

AU - ten Buren, E.B.J.

AU - Karrenbelt, M.A.P.

AU - Lingemann, M.

AU - Chordia, S.

AU - Deng, Y.

AU - Hu, J.J.

AU - Verest, J.M.

AU - Wu, V.

AU - Bello Gonzalez, T.D.G.

AU - van Heck, R.G.A.

AU - Odoni, D.I.

AU - Schonewille, T.

AU - van der Straat, L.

AU - de Graaff, L.H.

AU - van Passel, M.W.J.

PY - 2014

Y1 - 2014

N2 - Aspergillus niger is a filamentous fungus that is extensively used in industrial fermentations for protein expression and the production of organic acids. Inherent biosynthetic capabilities, such as the capacity to secrete these biomolecules in high amounts, make A. niger an attractive production host. Although A. niger is renowned for this ability, the knowledge of the molecular components that underlie its production capacity, intercellular trafficking processes and secretion mechanisms is far from complete. Here, we introduce a standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein. Expression of the GFP-actin construct facilitates visualization of the actin filaments of the cytoskeleton, whereas expression of the chromoprotein construct results in a clearly distinguishable red phenotype. These experimentally validated constructs constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena.

AB - Aspergillus niger is a filamentous fungus that is extensively used in industrial fermentations for protein expression and the production of organic acids. Inherent biosynthetic capabilities, such as the capacity to secrete these biomolecules in high amounts, make A. niger an attractive production host. Although A. niger is renowned for this ability, the knowledge of the molecular components that underlie its production capacity, intercellular trafficking processes and secretion mechanisms is far from complete. Here, we introduce a standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein. Expression of the GFP-actin construct facilitates visualization of the actin filaments of the cytoskeleton, whereas expression of the chromoprotein construct results in a clearly distinguishable red phenotype. These experimentally validated constructs constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena.

KW - gene

KW - hyphae

U2 - 10.1021/sb500304m

DO - 10.1021/sb500304m

M3 - Editorial

VL - 3

SP - 995

EP - 998

JO - ACS synthetic biology

T2 - ACS synthetic biology

JF - ACS synthetic biology

SN - 2161-5063

IS - 12

ER -

ten Buren EBJ, Karrenbelt MAP, Lingemann M, Chordia S, Deng Y, Hu JJ et al. Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger. ACS synthetic biology. 2014;3(12):995-998. https://doi.org/10.1021/sb500304m