TY - JOUR
T1 - Tomato spotted wilt virus transcriptase in vitro displays a preference for cap donors with multiple base complementary to the viral template
AU - van Knippenberg, I.C.
AU - Lamine, M.
AU - Goldbach, R.W.
AU - Kormelink, R.J.M.
PY - 2005
Y1 - 2005
N2 - Transcription of segmented negative-strand RNA viruses is initiated by cap snatching: a host mRNA is cleaved generally at 10¿20 nt from its 5¿ capped end and the resulting capped leader used to prime viral transcription. For Tomato spotted wilt virus (TSWV), type species of the plant-infecting Tospovirus genus within the Bunyaviridae, cap donors were previously shown to require a single base complementarity to the ultimate or penultimate viral template sequence. More recently, the occurrence in vitro of ¿re-snatching¿ of viral mRNAs, i.e., the use of viral mRNAs as cap donors, has been demonstrated for TSWV. To estimate the relative occurrence of re-snatching compared to snatching of host mRNAs, the use of cap donors with either single, double, or multiple complementarity to the viral template was analyzed in pair-wise competition in TSWV in vitro transcription assays. A strong preference was observed for multiple-basepairing donors
AB - Transcription of segmented negative-strand RNA viruses is initiated by cap snatching: a host mRNA is cleaved generally at 10¿20 nt from its 5¿ capped end and the resulting capped leader used to prime viral transcription. For Tomato spotted wilt virus (TSWV), type species of the plant-infecting Tospovirus genus within the Bunyaviridae, cap donors were previously shown to require a single base complementarity to the ultimate or penultimate viral template sequence. More recently, the occurrence in vitro of ¿re-snatching¿ of viral mRNAs, i.e., the use of viral mRNAs as cap donors, has been demonstrated for TSWV. To estimate the relative occurrence of re-snatching compared to snatching of host mRNAs, the use of cap donors with either single, double, or multiple complementarity to the viral template was analyzed in pair-wise competition in TSWV in vitro transcription assays. A strong preference was observed for multiple-basepairing donors
KW - messenger-rna synthesis
KW - influenza-virus
KW - 5' ends
KW - mosaic-virus
KW - independent translation
KW - heterogeneous sequences
KW - nsm protein
KW - s-rna
KW - polymerase
KW - bunyavirus
U2 - 10.1016/j.virol.2005.01.041
DO - 10.1016/j.virol.2005.01.041
M3 - Article
SN - 0042-6822
VL - 335
SP - 122
EP - 130
JO - Virology
JF - Virology
IS - 1
ER -