Tomato spotted wilt virus transcriptase in vitro displays a preference for cap donors with multiple base complementary to the viral template

I.C. van Knippenberg, M. Lamine, R.W. Goldbach, R.J.M. Kormelink

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)

Abstract

Transcription of segmented negative-strand RNA viruses is initiated by cap snatching: a host mRNA is cleaved generally at 10¿20 nt from its 5¿ capped end and the resulting capped leader used to prime viral transcription. For Tomato spotted wilt virus (TSWV), type species of the plant-infecting Tospovirus genus within the Bunyaviridae, cap donors were previously shown to require a single base complementarity to the ultimate or penultimate viral template sequence. More recently, the occurrence in vitro of ¿re-snatching¿ of viral mRNAs, i.e., the use of viral mRNAs as cap donors, has been demonstrated for TSWV. To estimate the relative occurrence of re-snatching compared to snatching of host mRNAs, the use of cap donors with either single, double, or multiple complementarity to the viral template was analyzed in pair-wise competition in TSWV in vitro transcription assays. A strong preference was observed for multiple-basepairing donors
Original languageEnglish
Pages (from-to)122-130
JournalVirology
Volume335
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • messenger-rna synthesis
  • influenza-virus
  • 5' ends
  • mosaic-virus
  • independent translation
  • heterogeneous sequences
  • nsm protein
  • s-rna
  • polymerase
  • bunyavirus

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