Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif

I.C. van Knippenberg; R.W. Goldbach; R.J.M. Kormelink

doi:10.1016/j.virusres.2005.01.012

Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif

I.C. van Knippenberg, R.W. Goldbach, R.J.M. Kormelink

Laboratory of Virology

Research output: Contribution to journal › Article › Academic › peer-review

30 Citations (Scopus)

Abstract

The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3¿-end of the intergenic hairpin, i.e. position 1568¿1574 for N and position 1852¿1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3¿-end. The potential involvement of the 3¿-end structure in transcription termination is discussed

Original language	English
Pages (from-to)	125-131
Journal	Virus Research
Volume	110
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virusres.2005.01.012
Publication status	Published - 2005

Keywords

mosaic-virus
independent translation
uukuniemi virus
punta-toro
viral-rna
termini
transcription
mechanism
protein
initiation

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title = "Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif",

abstract = "The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3¿-end of the intergenic hairpin, i.e. position 1568¿1574 for N and position 1852¿1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3¿-end. The potential involvement of the 3¿-end structure in transcription termination is discussed",

keywords = "mosaic-virus, independent translation, uukuniemi virus, punta-toro, viral-rna, termini, transcription, mechanism, protein, initiation",

author = "{van Knippenberg}, I.C. and R.W. Goldbach and R.J.M. Kormelink",

year = "2005",

doi = "10.1016/j.virusres.2005.01.012",

language = "English",

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pages = "125--131",

journal = "Virus Research",

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T1 - Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif

AU - van Knippenberg, I.C.

AU - Goldbach, R.W.

AU - Kormelink, R.J.M.

PY - 2005

Y1 - 2005

N2 - The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3¿-end of the intergenic hairpin, i.e. position 1568¿1574 for N and position 1852¿1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3¿-end. The potential involvement of the 3¿-end structure in transcription termination is discussed

AB - The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3¿-end of the intergenic hairpin, i.e. position 1568¿1574 for N and position 1852¿1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3¿-end. The potential involvement of the 3¿-end structure in transcription termination is discussed

KW - mosaic-virus

KW - independent translation

KW - uukuniemi virus

KW - punta-toro

KW - viral-rna

KW - termini

KW - transcription

KW - mechanism

KW - protein

KW - initiation

U2 - 10.1016/j.virusres.2005.01.012

DO - 10.1016/j.virusres.2005.01.012

M3 - Article

VL - 110

SP - 125

EP - 131

JO - Virus Research

JF - Virus Research

SN - 0168-1702

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