Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif

I.C. van Knippenberg, R.W. Goldbach, R.J.M. Kormelink

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)

Abstract

The Tomato spotted wilt virus ambisense M- and S-RNA segments contain an A/U-rich intergenic region predicted to form a stable hairpin structure. The site of transcription termination of S-segment encoded N and NSs mRNAs synthesised in an in vitro transcription system was roughly mapped to the 3¿-end of the intergenic hairpin, i.e. position 1568¿1574 for N and position 1852¿1839 for NSs, as determined by RT-PCR cloning and size estimation on Northern blots. This suggests that these viral transcripts contain a predicted stem-loop structure at their 3¿-end. The potential involvement of the 3¿-end structure in transcription termination is discussed
Original languageEnglish
Pages (from-to)125-131
JournalVirus Research
Volume110
Issue number1-2
DOIs
Publication statusPublished - 2005

Keywords

  • mosaic-virus
  • independent translation
  • uukuniemi virus
  • punta-toro
  • viral-rna
  • termini
  • transcription
  • mechanism
  • protein
  • initiation

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