Tomato spotted wilt virus nucleocapsid protein interacts with both viral glycoproteins Gn and Gc in planta

D.M.O.G. Ribeiro, J.W. Borst, R.W. Goldbach, R.J.M. Kormelink

Research output: Contribution to journalArticleAcademicpeer-review

51 Citations (Scopus)

Abstract

Recently, the Tomato Spotted Wilt Virus (TSWV) Gn and Gc glycoproteins were shown to induce the formation of (pseudo-) circular and pleomorphic membrane structures upon transient expression in plant cells. Furthermore, when singly expressed, Gc retains in the ER, while Gn is able to further migrate to the Golgi. Upon co-expression, Gn rescues Gc and co-migrates to the Golgi complex. Here, we have studied the behavior of the glycoproteins in the presence of the viral nucleocapsid (N) protein and in vivo analyzed the occurrence of protein¿protein interactions by fluorescence life time imaging microscopy (FLIM). The analysis demonstrated that N co-localizes and interacts with both glycoproteins, with a preference for Gn. Additionally, it is shown that N causes a dramatic change in the distribution of Gc within the ER, from reticular to punctate spots. The observations are discussed in the context of the virus particle formation during the infection process.
Original languageEnglish
Pages (from-to)121-130
JournalVirology
Volume383
Issue number1
DOIs
Publication statusPublished - 2009

Keywords

  • endoplasmic-reticulum
  • homotypic interaction
  • cells
  • golgi
  • localization
  • microscopy
  • infection
  • tobacco

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