Toll-Like Receptor 2 Activation by beta 2 -> 1-Fructans Protects Barrier Function of T84 Human Intestinal Epithelial Cells in a Chain Length-Dependent Manner

L.M. Vogt, D. Meyer, G. Pullens, M.M. Faas, K. Venema, U. Ramasamy, H.A. Schols, P. Vos

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44 Citations (Scopus)

Abstract

Dietary fiber intake is associated with lower incidence and mortality from disease, but the underlying mechanisms of these protective effects are unclear. We hypothesized that beta 2 -> 1-fructan dietary fibers confer protection on intestinal epithelial cell barrier function via Toll-like receptor 2 (TLR2), and we studied whether beta 2 -> 1-fructan chain-length differences affect this process. T84 human intestinal epithelial cell monolayers were incubated with 4 beta 2 -> 1-fructan formulations of different chain-length compositions and were stimulated with the proinflammatory phorbol 12-myristate 13-acetate (PMA). Transepithelial electrical resistance (TEER) was analyzed by electric cell substrate impedance sensing (ECIS) as a measure for tight junction-mediated barrier function. To confirm TLR2 involvement in barrier modulation by beta 2 -> 1-fructans, ECIS experiments were repeated using TLR2 blocking antibody. After preincubation of T84 cells with short-chain beta 2 -> 1-fructans, the decrease in TEER as induced by PMA (62.3 +/- 5.2%, P <0.001) was strongly attenuated (15.2 8.8%, P <0.01). However, when PMA was applied first, no effect on recovery was observed during addition of the fructans. By blocking TLR2 on the T84 cells, the protective effect of short-chain beta 2 -> 1-fructans was substantially inhibited. Stimulation of human embryonic kidney human TLR2 reporter cells with beta 2 -> 1-fructans induced activation of nuclear factor kappa-light-chain-enhancer of activated B cells, confirming that beta 2 -> 1-fructans are specific ligands for TLR2. To conclude, beta 2 -> 1-fructans exert time-dependent and chain length-dependent protective effects on the T84 intestinal epithelial cell barrier mediated via TLR2. These results suggest that TLR2 located on intestinal epithelial cells could be a target of beta 2 -> 1-fructan-mediated health effects.
Original languageEnglish
Pages (from-to)1002-1008
JournalThe Journal of Nutrition
Volume144
Issue number7
DOIs
Publication statusPublished - 2014

Keywords

  • cerebral ischemia/reperfusion injury
  • kinase-c isoforms
  • dietary fiber
  • bronchial-asthma
  • tyrosine kinase
  • dendritic cells
  • gut microbiota
  • celiac-disease
  • fatty-acids
  • pkc-alpha

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