The ambition of the Sustainable Dairy Chain (DZK) is to map out and promote biodiversity on all dairy farms in the Netherlands by means of the ‘Biodiversity Monitor’. This report describes the results of an assessment of the selected Key Performance Indicators (KPIs) against biodiversity measurements on dairy farms. The percentage of grassland and the percentage of permanent grassland can be used as KPI for biodiversity at farm level. The KPI’s nitrogen soil surplus per ha and percentage of feed protein from own farms are related to a number of biodiversity measurements. Depending on the biodiversity target, these KPIs can be used to a greater or lesser extent as an indicator of biodiversity on a farm.