To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

M. Aguirre; J. Ramiro Garcia; M.E. Koenen; K. Venema

doi:10.1016/j.mimet.2014.08.022

To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

M. Aguirre, J. Ramiro Garcia, M.E. Koenen, K. Venema

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.

Original language	English
Pages (from-to)	1-7
Journal	Journal of Microbiological Methods
Volume	107
DOIs	https://doi.org/10.1016/j.mimet.2014.08.022
Publication status	Published - 2014

Keywords

human large-intestine
gut microbiota
model
bacteria
oligosaccharides
metabolism
products
patterns
starch
core

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10.1016/j.mimet.2014.08.022

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title = "To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies",

abstract = "This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.",

keywords = "human large-intestine, gut microbiota, model, bacteria, oligosaccharides, metabolism, products, patterns, starch, core",

author = "M. Aguirre and {Ramiro Garcia}, J. and M.E. Koenen and K. Venema",

year = "2014",

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AU - Aguirre, M.

AU - Ramiro Garcia, J.

AU - Koenen, M.E.

AU - Venema, K.

PY - 2014

Y1 - 2014

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AB - This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.

KW - human large-intestine

KW - gut microbiota

KW - model

KW - bacteria

KW - oligosaccharides

KW - metabolism

KW - products

KW - patterns

KW - starch

KW - core

U2 - 10.1016/j.mimet.2014.08.022

DO - 10.1016/j.mimet.2014.08.022

M3 - Article

SN - 0167-7012

VL - 107

SP - 1

EP - 7

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

Abstract

Keywords

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