Tissue-specific expression of the beta-glucuronidase reporter gene in transgenic strawberry (Fragaria Xananassa) plants

    Research output: Contribution to journalArticleAcademicpeer-review

    42 Citations (Scopus)

    Abstract

    The strawberry ( Fragaria spp) is regarded as a false fruit because it originates from the receptacle, which is a non-ovarian tissue. For this reason, fruit-specific promoters isolated from plant species in which the fruit is derived from the ovary wall might not be suited to control gene expression in a fruit-specific way in strawberry. In order to achieve (false) fruit-specific expression in strawberry, we tested the petunia FBP7 (floral binding protein7) promoter, which proved to be active in the receptacles of petunia flowers, in transgenic strawberry fruits. In strawberry plants containing the FBP7 promoter fused to the ß-glucuronidase (GUS) reporter gene ( gus), GUS activity was found in floral and fruit tissues of all developmental stages tested but not in leaf, petiole and root tissue . Surprisingly, Northern blot analysis showed the presence of gus-derived mRNAs in root (strong) and petiole (weak) tissue of fbp7- gus plants in addition to the floral and fruit tissues. Therefore, it is concluded that the histological GUS phenotype does not necessarily correspond with expression at the mRNA level. mRNA quantification using the TaqMan polymerase chain reaction technology confirmed the Northern results and showed that in red strawberry tissue the cauliflower mosaic virus 35S promoter is at least sixfold stronger than the FBP7 promoter
    Original languageEnglish
    Pages (from-to)313-319
    JournalPlant Cell Reports
    Volume21
    Issue number4
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Fragaria
    beta-glucuronidase
    reporter genes
    strawberries
    genetically modified organisms
    fruits
    promoter regions
    Petunia
    tissues
    Cauliflower mosaic virus
    Northern blotting
    polymerase chain reaction
    developmental stages
    flowers
    phenotype
    gene expression

    Keywords

    • mediated transformation
    • agrobacterium
    • ethylene
    • fruit

    Cite this

    @article{8ee5a7dcfc4a4c6bb52f3dfb2160f7c0,
    title = "Tissue-specific expression of the beta-glucuronidase reporter gene in transgenic strawberry (Fragaria Xananassa) plants",
    abstract = "The strawberry ( Fragaria spp) is regarded as a false fruit because it originates from the receptacle, which is a non-ovarian tissue. For this reason, fruit-specific promoters isolated from plant species in which the fruit is derived from the ovary wall might not be suited to control gene expression in a fruit-specific way in strawberry. In order to achieve (false) fruit-specific expression in strawberry, we tested the petunia FBP7 (floral binding protein7) promoter, which proved to be active in the receptacles of petunia flowers, in transgenic strawberry fruits. In strawberry plants containing the FBP7 promoter fused to the {\ss}-glucuronidase (GUS) reporter gene ( gus), GUS activity was found in floral and fruit tissues of all developmental stages tested but not in leaf, petiole and root tissue . Surprisingly, Northern blot analysis showed the presence of gus-derived mRNAs in root (strong) and petiole (weak) tissue of fbp7- gus plants in addition to the floral and fruit tissues. Therefore, it is concluded that the histological GUS phenotype does not necessarily correspond with expression at the mRNA level. mRNA quantification using the TaqMan polymerase chain reaction technology confirmed the Northern results and showed that in red strawberry tissue the cauliflower mosaic virus 35S promoter is at least sixfold stronger than the FBP7 promoter",
    keywords = "mediated transformation, agrobacterium, ethylene, fruit",
    author = "J.G. Schaart and E.M.J. Salentijn and F.A. Krens",
    year = "2002",
    doi = "10.1007/s00299-002-0514-4",
    language = "English",
    volume = "21",
    pages = "313--319",
    journal = "Plant Cell Reports",
    issn = "0721-7714",
    publisher = "Springer Verlag",
    number = "4",

    }

    Tissue-specific expression of the beta-glucuronidase reporter gene in transgenic strawberry (Fragaria Xananassa) plants. / Schaart, J.G.; Salentijn, E.M.J.; Krens, F.A.

    In: Plant Cell Reports, Vol. 21, No. 4, 2002, p. 313-319.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Tissue-specific expression of the beta-glucuronidase reporter gene in transgenic strawberry (Fragaria Xananassa) plants

    AU - Schaart, J.G.

    AU - Salentijn, E.M.J.

    AU - Krens, F.A.

    PY - 2002

    Y1 - 2002

    N2 - The strawberry ( Fragaria spp) is regarded as a false fruit because it originates from the receptacle, which is a non-ovarian tissue. For this reason, fruit-specific promoters isolated from plant species in which the fruit is derived from the ovary wall might not be suited to control gene expression in a fruit-specific way in strawberry. In order to achieve (false) fruit-specific expression in strawberry, we tested the petunia FBP7 (floral binding protein7) promoter, which proved to be active in the receptacles of petunia flowers, in transgenic strawberry fruits. In strawberry plants containing the FBP7 promoter fused to the ß-glucuronidase (GUS) reporter gene ( gus), GUS activity was found in floral and fruit tissues of all developmental stages tested but not in leaf, petiole and root tissue . Surprisingly, Northern blot analysis showed the presence of gus-derived mRNAs in root (strong) and petiole (weak) tissue of fbp7- gus plants in addition to the floral and fruit tissues. Therefore, it is concluded that the histological GUS phenotype does not necessarily correspond with expression at the mRNA level. mRNA quantification using the TaqMan polymerase chain reaction technology confirmed the Northern results and showed that in red strawberry tissue the cauliflower mosaic virus 35S promoter is at least sixfold stronger than the FBP7 promoter

    AB - The strawberry ( Fragaria spp) is regarded as a false fruit because it originates from the receptacle, which is a non-ovarian tissue. For this reason, fruit-specific promoters isolated from plant species in which the fruit is derived from the ovary wall might not be suited to control gene expression in a fruit-specific way in strawberry. In order to achieve (false) fruit-specific expression in strawberry, we tested the petunia FBP7 (floral binding protein7) promoter, which proved to be active in the receptacles of petunia flowers, in transgenic strawberry fruits. In strawberry plants containing the FBP7 promoter fused to the ß-glucuronidase (GUS) reporter gene ( gus), GUS activity was found in floral and fruit tissues of all developmental stages tested but not in leaf, petiole and root tissue . Surprisingly, Northern blot analysis showed the presence of gus-derived mRNAs in root (strong) and petiole (weak) tissue of fbp7- gus plants in addition to the floral and fruit tissues. Therefore, it is concluded that the histological GUS phenotype does not necessarily correspond with expression at the mRNA level. mRNA quantification using the TaqMan polymerase chain reaction technology confirmed the Northern results and showed that in red strawberry tissue the cauliflower mosaic virus 35S promoter is at least sixfold stronger than the FBP7 promoter

    KW - mediated transformation

    KW - agrobacterium

    KW - ethylene

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