Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)

Milica Bogdanovic, K. Cankar, Sladana Todorovic, Milan Dragicevic, Ana Simonovic, A.M.M.L. van Houwelingen, E.G.W.M. Schijlen, A. Schipper, David Gagneul, Theo Hendriks, Marie-Christine Quillet, H.J. Bouwmeester, H.J. Bosch, M.J. Beekwilder

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis of
leaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.
Original languageEnglish
Pages (from-to)253-260
JournalIndustrial Crops and Products
Volume129
DOIs
Publication statusPublished - Mar 2019

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Cichorium intybus
chicory
sesquiterpenoid lactones
Asteraceae
biosynthesis
genomics
germacrene
epidermis (plant)
biochemical pathways
germplasm
genes
physiology
promoter regions
tissues
clones
gene expression
enzymes

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Bogdanovic, Milica ; Cankar, K. ; Todorovic, Sladana ; Dragicevic, Milan ; Simonovic, Ana ; van Houwelingen, A.M.M.L. ; Schijlen, E.G.W.M. ; Schipper, A. ; Gagneul, David ; Hendriks, Theo ; Quillet, Marie-Christine ; Bouwmeester, H.J. ; Bosch, H.J. ; Beekwilder, M.J. / Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae). In: Industrial Crops and Products. 2019 ; Vol. 129. pp. 253-260.
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title = "Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)",
abstract = "Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis ofleaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.",
author = "Milica Bogdanovic and K. Cankar and Sladana Todorovic and Milan Dragicevic and Ana Simonovic and {van Houwelingen}, A.M.M.L. and E.G.W.M. Schijlen and A. Schipper and David Gagneul and Theo Hendriks and Marie-Christine Quillet and H.J. Bouwmeester and H.J. Bosch and M.J. Beekwilder",
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Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae). / Bogdanovic, Milica; Cankar, K.; Todorovic, Sladana; Dragicevic, Milan; Simonovic, Ana; van Houwelingen, A.M.M.L.; Schijlen, E.G.W.M.; Schipper, A.; Gagneul, David; Hendriks, Theo; Quillet, Marie-Christine; Bouwmeester, H.J.; Bosch, H.J.; Beekwilder, M.J.

In: Industrial Crops and Products, Vol. 129, 03.2019, p. 253-260.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)

AU - Bogdanovic, Milica

AU - Cankar, K.

AU - Todorovic, Sladana

AU - Dragicevic, Milan

AU - Simonovic, Ana

AU - van Houwelingen, A.M.M.L.

AU - Schijlen, E.G.W.M.

AU - Schipper, A.

AU - Gagneul, David

AU - Hendriks, Theo

AU - Quillet, Marie-Christine

AU - Bouwmeester, H.J.

AU - Bosch, H.J.

AU - Beekwilder, M.J.

PY - 2019/3

Y1 - 2019/3

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AB - Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis ofleaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.

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DO - 10.1016/j.indcrop.2018.12.011

M3 - Article

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EP - 260

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

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