Tissue location of resistance in apple to the rosy apple aphid established by electrical penetration graphs

E. Marchetti, S. Civolani, M. Leis, M. Chicca, W.F. Tjallingii, E. Pasqualini, P. Baroni

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

A study of the constitutive resistance of the apple cultivar Florina, Malus domestica Borkh. (Rosaceae), to the rosy apple aphid, Dysaphis plantaginea (Passerini) (Homoptera Aphididae), was performed for the first time by the electrical penetration graph (DC-EPG) system, using the susceptible apple cultivar Smoothe as control. All experiments were conducted with apterous adult virginoparae. The results showed a constitutive resistance in Florina due to a much longer period before the first probe reflecting surface factors. Some weak indications were found for pre-phloem resistance and initiating phloem access was not affected as inferred from equal time to show phloem salivation. However, the complete absence of phloem ingestion indicates a major resistance factor in the phloem sieve elements, most likely in the sieve element sap. Surface factors could have affected tissue related variables and this should be studied further. Anyhow, the strong constitutive resistance in Florina, either on the surface alone or in the phloem as well, effectively prevented reliable experiments on induced resistance, previously detected by molecular methods.
Original languageEnglish
Pages (from-to)203-208
JournalBulletin of Insectology
Volume62
Issue number2
Publication statusPublished - 2009

Fingerprint

Dysaphis plantaginea
phloem
apples
sieve elements
salivation
induced resistance
Rosaceae
cultivars
Malus domestica
Aphididae
Homoptera
sap
tissues
ingestion

Keywords

  • dysaphis-plantaginea
  • stylet penetration
  • malus-domestica
  • myzus-persicae
  • inoculation
  • salivation
  • cultivars
  • homoptera
  • viruses

Cite this

Marchetti, E., Civolani, S., Leis, M., Chicca, M., Tjallingii, W. F., Pasqualini, E., & Baroni, P. (2009). Tissue location of resistance in apple to the rosy apple aphid established by electrical penetration graphs. Bulletin of Insectology, 62(2), 203-208.
Marchetti, E. ; Civolani, S. ; Leis, M. ; Chicca, M. ; Tjallingii, W.F. ; Pasqualini, E. ; Baroni, P. / Tissue location of resistance in apple to the rosy apple aphid established by electrical penetration graphs. In: Bulletin of Insectology. 2009 ; Vol. 62, No. 2. pp. 203-208.
@article{289a5a129d5742f999fc8440fac27a12,
title = "Tissue location of resistance in apple to the rosy apple aphid established by electrical penetration graphs",
abstract = "A study of the constitutive resistance of the apple cultivar Florina, Malus domestica Borkh. (Rosaceae), to the rosy apple aphid, Dysaphis plantaginea (Passerini) (Homoptera Aphididae), was performed for the first time by the electrical penetration graph (DC-EPG) system, using the susceptible apple cultivar Smoothe as control. All experiments were conducted with apterous adult virginoparae. The results showed a constitutive resistance in Florina due to a much longer period before the first probe reflecting surface factors. Some weak indications were found for pre-phloem resistance and initiating phloem access was not affected as inferred from equal time to show phloem salivation. However, the complete absence of phloem ingestion indicates a major resistance factor in the phloem sieve elements, most likely in the sieve element sap. Surface factors could have affected tissue related variables and this should be studied further. Anyhow, the strong constitutive resistance in Florina, either on the surface alone or in the phloem as well, effectively prevented reliable experiments on induced resistance, previously detected by molecular methods.",
keywords = "dysaphis-plantaginea, stylet penetration, malus-domestica, myzus-persicae, inoculation, salivation, cultivars, homoptera, viruses",
author = "E. Marchetti and S. Civolani and M. Leis and M. Chicca and W.F. Tjallingii and E. Pasqualini and P. Baroni",
note = "09-3263",
year = "2009",
language = "English",
volume = "62",
pages = "203--208",
journal = "Bulletin of Insectology",
issn = "1721-8861",
publisher = "Universit{\`a} di Bologna, Department of Agricultural Sciences",
number = "2",

}

Marchetti, E, Civolani, S, Leis, M, Chicca, M, Tjallingii, WF, Pasqualini, E & Baroni, P 2009, 'Tissue location of resistance in apple to the rosy apple aphid established by electrical penetration graphs' Bulletin of Insectology, vol. 62, no. 2, pp. 203-208.

Tissue location of resistance in apple to the rosy apple aphid established by electrical penetration graphs. / Marchetti, E.; Civolani, S.; Leis, M.; Chicca, M.; Tjallingii, W.F.; Pasqualini, E.; Baroni, P.

In: Bulletin of Insectology, Vol. 62, No. 2, 2009, p. 203-208.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Tissue location of resistance in apple to the rosy apple aphid established by electrical penetration graphs

AU - Marchetti, E.

AU - Civolani, S.

AU - Leis, M.

AU - Chicca, M.

AU - Tjallingii, W.F.

AU - Pasqualini, E.

AU - Baroni, P.

N1 - 09-3263

PY - 2009

Y1 - 2009

N2 - A study of the constitutive resistance of the apple cultivar Florina, Malus domestica Borkh. (Rosaceae), to the rosy apple aphid, Dysaphis plantaginea (Passerini) (Homoptera Aphididae), was performed for the first time by the electrical penetration graph (DC-EPG) system, using the susceptible apple cultivar Smoothe as control. All experiments were conducted with apterous adult virginoparae. The results showed a constitutive resistance in Florina due to a much longer period before the first probe reflecting surface factors. Some weak indications were found for pre-phloem resistance and initiating phloem access was not affected as inferred from equal time to show phloem salivation. However, the complete absence of phloem ingestion indicates a major resistance factor in the phloem sieve elements, most likely in the sieve element sap. Surface factors could have affected tissue related variables and this should be studied further. Anyhow, the strong constitutive resistance in Florina, either on the surface alone or in the phloem as well, effectively prevented reliable experiments on induced resistance, previously detected by molecular methods.

AB - A study of the constitutive resistance of the apple cultivar Florina, Malus domestica Borkh. (Rosaceae), to the rosy apple aphid, Dysaphis plantaginea (Passerini) (Homoptera Aphididae), was performed for the first time by the electrical penetration graph (DC-EPG) system, using the susceptible apple cultivar Smoothe as control. All experiments were conducted with apterous adult virginoparae. The results showed a constitutive resistance in Florina due to a much longer period before the first probe reflecting surface factors. Some weak indications were found for pre-phloem resistance and initiating phloem access was not affected as inferred from equal time to show phloem salivation. However, the complete absence of phloem ingestion indicates a major resistance factor in the phloem sieve elements, most likely in the sieve element sap. Surface factors could have affected tissue related variables and this should be studied further. Anyhow, the strong constitutive resistance in Florina, either on the surface alone or in the phloem as well, effectively prevented reliable experiments on induced resistance, previously detected by molecular methods.

KW - dysaphis-plantaginea

KW - stylet penetration

KW - malus-domestica

KW - myzus-persicae

KW - inoculation

KW - salivation

KW - cultivars

KW - homoptera

KW - viruses

M3 - Article

VL - 62

SP - 203

EP - 208

JO - Bulletin of Insectology

JF - Bulletin of Insectology

SN - 1721-8861

IS - 2

ER -

Marchetti E, Civolani S, Leis M, Chicca M, Tjallingii WF, Pasqualini E et al. Tissue location of resistance in apple to the rosy apple aphid established by electrical penetration graphs. Bulletin of Insectology. 2009;62(2):203-208.