Three genes identified by genome wide association mapping of root-knot nematode susceptibility in Arabidopsis

M.G. Sterken; S. Warmerdam; C.C. van Schaik; M.E.P. Oortwijn; J.L. Lozano Torres; J. Bakker; G. Smant

Abstract

Root-knot nematodes are devastating plant-pathogens affecting agricultural productivity of major food crops world-wide. Current control strategies depend on a limited supply of nematode resistance genes. Hence, there is need for alternative control strategies. Here, we show that more subtle quantitative trait loci could be used as additional genetic resource for nematode control.

We mapped the genetic architecture of susceptibility in 340 natural isolates of Arabidopsis thaliana to Meloidogyne incognitia. We found ample genetic variation in M. incognita reproduction on these plants, with a narrow-sense heritability of 0.52. By genome wide association 19 associated loci were identified. Three loci were investigated by characterizing nine T-DNA knock-out mutants of candidate genes, identifying three (co-)regulators of nematode susceptibility in Arabidopsis: BZR1, FRNI1, and ERF6.

Our results suggest that allelic variation in susceptibility genes could be used to improve nematode resistance of plants, paving the way to investigate these traits in food-crops.

Original language	English
Publication status	Published - May 2019
Event	7th plant genomics & gene editing congress: Europe - WTC, Rotterdam, Netherlands Duration: 21 May 2019 → 22 May 2019

Conference

Conference	7th plant genomics & gene editing congress: Europe
Country	Netherlands
City	Rotterdam
Period	21/05/19 → 22/05/19

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@conference{a160065fa57e4cd697265d2233eac49f,

title = "Three genes identified by genome wide association mapping of root-knot nematode susceptibility in Arabidopsis",

abstract = "Root-knot nematodes are devastating plant-pathogens affecting agricultural productivity of major food crops world-wide. Current control strategies depend on a limited supply of nematode resistance genes. Hence, there is need for alternative control strategies. Here, we show that more subtle quantitative trait loci could be used as additional genetic resource for nematode control.We mapped the genetic architecture of susceptibility in 340 natural isolates of Arabidopsis thaliana to Meloidogyne incognitia. We found ample genetic variation in M. incognita reproduction on these plants, with a narrow-sense heritability of 0.52. By genome wide association 19 associated loci were identified. Three loci were investigated by characterizing nine T-DNA knock-out mutants of candidate genes, identifying three (co-)regulators of nematode susceptibility in Arabidopsis: BZR1, FRNI1, and ERF6.Our results suggest that allelic variation in susceptibility genes could be used to improve nematode resistance of plants, paving the way to investigate these traits in food-crops.",

author = "M.G. Sterken and S. Warmerdam and {van Schaik}, C.C. and M.E.P. Oortwijn and {Lozano Torres}, J.L. and J. Bakker and G. Smant",

year = "2019",

month = may,

language = "English",

note = "7th plant genomics & gene editing congress: Europe ; Conference date: 21-05-2019 Through 22-05-2019",

}

Three genes identified by genome wide association mapping of root-knot nematode susceptibility in Arabidopsis. / Sterken, M.G.; Warmerdam, S.; van Schaik, C.C.; Oortwijn, M.E.P.; Lozano Torres, J.L.; Bakker, J.; Smant, G.

2019. Abstract from 7th plant genomics & gene editing congress: Europe, Rotterdam, Netherlands.

Research output: Contribution to conference › Abstract

TY - CONF

T1 - Three genes identified by genome wide association mapping of root-knot nematode susceptibility in Arabidopsis

AU - Sterken, M.G.

AU - Warmerdam, S.

AU - van Schaik, C.C.

AU - Oortwijn, M.E.P.

AU - Lozano Torres, J.L.

AU - Bakker, J.

AU - Smant, G.

PY - 2019/5

Y1 - 2019/5

N2 - Root-knot nematodes are devastating plant-pathogens affecting agricultural productivity of major food crops world-wide. Current control strategies depend on a limited supply of nematode resistance genes. Hence, there is need for alternative control strategies. Here, we show that more subtle quantitative trait loci could be used as additional genetic resource for nematode control.We mapped the genetic architecture of susceptibility in 340 natural isolates of Arabidopsis thaliana to Meloidogyne incognitia. We found ample genetic variation in M. incognita reproduction on these plants, with a narrow-sense heritability of 0.52. By genome wide association 19 associated loci were identified. Three loci were investigated by characterizing nine T-DNA knock-out mutants of candidate genes, identifying three (co-)regulators of nematode susceptibility in Arabidopsis: BZR1, FRNI1, and ERF6.Our results suggest that allelic variation in susceptibility genes could be used to improve nematode resistance of plants, paving the way to investigate these traits in food-crops.

AB - Root-knot nematodes are devastating plant-pathogens affecting agricultural productivity of major food crops world-wide. Current control strategies depend on a limited supply of nematode resistance genes. Hence, there is need for alternative control strategies. Here, we show that more subtle quantitative trait loci could be used as additional genetic resource for nematode control.We mapped the genetic architecture of susceptibility in 340 natural isolates of Arabidopsis thaliana to Meloidogyne incognitia. We found ample genetic variation in M. incognita reproduction on these plants, with a narrow-sense heritability of 0.52. By genome wide association 19 associated loci were identified. Three loci were investigated by characterizing nine T-DNA knock-out mutants of candidate genes, identifying three (co-)regulators of nematode susceptibility in Arabidopsis: BZR1, FRNI1, and ERF6.Our results suggest that allelic variation in susceptibility genes could be used to improve nematode resistance of plants, paving the way to investigate these traits in food-crops.

M3 - Abstract

T2 - 7th plant genomics & gene editing congress: Europe

Y2 - 21 May 2019 through 22 May 2019

ER -