Thioflavin T and Birefringence Assays to Determine the Conversion of Proteins into Fibrils

S.G. Bolder; L.M.C. Sagis; P. Venema; E. van der Linden

doi:10.1021/la063048k

Thioflavin T and Birefringence Assays to Determine the Conversion of Proteins into Fibrils

S.G. Bolder, L.M.C. Sagis, P. Venema, E. van der Linden

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The conversion of protein monomers into fibrils can be determined using the centrifugal filtration method. The results of this method were used to calibrate steady-shear birefringence and Thioflavin T fluorescence measurements. For both measurements, a linear correlation with the fibril concentration was extracted, resulting in two fast assays to determine the fibril concentration quantitatively. From birefringence measurements and the conversion determined using the centrifugal filtration method, we were able to calculate more precise values for the birefringence per unit length of the fibrils (M) and the flexibility of the fibrils (ß)

Original language	English
Pages (from-to)	4144-4147
Journal	Langmuir
Volume	23
DOIs	https://doi.org/10.1021/la063048k
Publication status	Published - 2007

Keywords

beta-lactoglobulin gels
amyloid fibrils
whey proteins
low ph
aggregation
fibrillation
fabrication
mechanism
gelation
route

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title = "Thioflavin T and Birefringence Assays to Determine the Conversion of Proteins into Fibrils",

abstract = "The conversion of protein monomers into fibrils can be determined using the centrifugal filtration method. The results of this method were used to calibrate steady-shear birefringence and Thioflavin T fluorescence measurements. For both measurements, a linear correlation with the fibril concentration was extracted, resulting in two fast assays to determine the fibril concentration quantitatively. From birefringence measurements and the conversion determined using the centrifugal filtration method, we were able to calculate more precise values for the birefringence per unit length of the fibrils (M) and the flexibility of the fibrils ({\ss})",

keywords = "beta-lactoglobulin gels, amyloid fibrils, whey proteins, low ph, aggregation, fibrillation, fabrication, mechanism, gelation, route",

author = "S.G. Bolder and L.M.C. Sagis and P. Venema and {van der Linden}, E.",

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year = "2007",

doi = "10.1021/la063048k",

language = "English",

volume = "23",

pages = "4144--4147",

journal = "Langmuir",

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T1 - Thioflavin T and Birefringence Assays to Determine the Conversion of Proteins into Fibrils

AU - Bolder, S.G.

AU - Sagis, L.M.C.

AU - Venema, P.

AU - van der Linden, E.

N1 - F832

PY - 2007

Y1 - 2007

N2 - The conversion of protein monomers into fibrils can be determined using the centrifugal filtration method. The results of this method were used to calibrate steady-shear birefringence and Thioflavin T fluorescence measurements. For both measurements, a linear correlation with the fibril concentration was extracted, resulting in two fast assays to determine the fibril concentration quantitatively. From birefringence measurements and the conversion determined using the centrifugal filtration method, we were able to calculate more precise values for the birefringence per unit length of the fibrils (M) and the flexibility of the fibrils (ß)

AB - The conversion of protein monomers into fibrils can be determined using the centrifugal filtration method. The results of this method were used to calibrate steady-shear birefringence and Thioflavin T fluorescence measurements. For both measurements, a linear correlation with the fibril concentration was extracted, resulting in two fast assays to determine the fibril concentration quantitatively. From birefringence measurements and the conversion determined using the centrifugal filtration method, we were able to calculate more precise values for the birefringence per unit length of the fibrils (M) and the flexibility of the fibrils (ß)

KW - beta-lactoglobulin gels

KW - amyloid fibrils

KW - whey proteins

KW - low ph

KW - aggregation

KW - fibrillation

KW - fabrication

KW - mechanism

KW - gelation

KW - route

U2 - 10.1021/la063048k

DO - 10.1021/la063048k

M3 - Article

SN - 0743-7463

VL - 23

SP - 4144

EP - 4147

JO - Langmuir

JF - Langmuir

ER -

Thioflavin T and Birefringence Assays to Determine the Conversion of Proteins into Fibrils

Abstract

Keywords

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