Thermostable Cas9 nucleases

J. van der Oost; R. van Kranenburg; E.F. Bosma; I. Mougiakos; P. Mohanraju

Thermostable Cas9 nucleases

J. van der Oost (Inventor), R. van Kranenburg (Inventor), E.F. Bosma (Inventor), I. Mougiakos (Inventor), P. Mohanraju (Inventor)

Research output: Patent

Abstract

The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

Original language	English
Patent number	WO2018108339
Priority date	14/12/16
Publication status	Published - 21 Jun 2018

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1 Patent

Thermostable Cas9 nucleases
van der Oost, J. (Inventor), Daas, M. J. A. (Inventor), Kengen, S. W. M. (Inventor) & de Vos, W. M. (Inventor), 15 Dec 2016, Patent No. WO2016198361, Priority date 12 Jun 2015
Research output: Patent

Open Access

Cite this

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title = "Thermostable Cas9 nucleases",

abstract = "The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.",

author = "{van der Oost}, J. and {van Kranenburg}, R. and E.F. Bosma and I. Mougiakos and P. Mohanraju",

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T1 - Thermostable Cas9 nucleases

AU - van der Oost, J.

AU - van Kranenburg, R.

AU - Bosma, E.F.

AU - Mougiakos, I.

AU - Mohanraju, P.

PY - 2018/6/21

Y1 - 2018/6/21

N2 - The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

AB - The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

M3 - Patent

M1 - WO2018108339

ER -

Thermostable Cas9 nucleases

Abstract

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Research output

Thermostable Cas9 nucleases

Cite this