Thermostable Cas9 nucleases

J. van der Oost (Inventor), R. van Kranenburg (Inventor), E.F. Bosma (Inventor), I. Mougiakos (Inventor), P. Mohanraju (Inventor)

Research output: PatentOther research output

Abstract

The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.
Original languageEnglish
Patent numberWO2018108339
Priority date14/12/16
Publication statusPublished - 21 Jun 2018

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Micrococcal Nuclease
Genetic Engineering
Nucleic Acids
Peptides
Polynucleotides
Proteins
Temperature
Amino Acid Sequence

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title = "Thermostable Cas9 nucleases",
abstract = "The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77{\%} identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.",
author = "{van der Oost}, J. and {van Kranenburg}, R. and E.F. Bosma and I. Mougiakos and P. Mohanraju",
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day = "21",
language = "English",
type = "Patent",
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Thermostable Cas9 nucleases. / van der Oost, J. (Inventor); van Kranenburg, R. (Inventor); Bosma, E.F. (Inventor); Mougiakos, I. (Inventor); Mohanraju, P. (Inventor).

Patent No.: WO2018108339.

Research output: PatentOther research output

TY - PAT

T1 - Thermostable Cas9 nucleases

AU - van der Oost, J.

AU - van Kranenburg, R.

AU - Bosma, E.F.

AU - Mougiakos, I.

AU - Mohanraju, P.

PY - 2018/6/21

Y1 - 2018/6/21

N2 - The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

AB - The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

M3 - Patent

M1 - WO2018108339

ER -