Thermostable Cas9 nucleases

J. van der Oost (Inventor), M.J.A. Daas (Inventor), S.W.M. Kengen (Inventor), W.M. de Vos (Inventor)

Research output: PatentOther research output

Abstract

Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
Original languageEnglish
Patent numberWO2016198361
Priority date12/06/15
Publication statusPublished - 15 Dec 2016

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Micrococcal Nuclease
Genetic Engineering
Nucleic Acids
DNA Cleavage
Peptides
Temperature
Amino Acid Sequence
Proteins
Gene Editing

Cite this

van der Oost, J., Daas, M. J. A., Kengen, S. W. M., & de Vos, W. M. (2016). Thermostable Cas9 nucleases. (Patent No. WO2016198361).
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abstract = "Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77{\%} identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.",
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Thermostable Cas9 nucleases. / van der Oost, J. (Inventor); Daas, M.J.A. (Inventor); Kengen, S.W.M. (Inventor); de Vos, W.M. (Inventor).

Patent No.: WO2016198361.

Research output: PatentOther research output

TY - PAT

T1 - Thermostable Cas9 nucleases

AU - van der Oost, J.

AU - Daas, M.J.A.

AU - Kengen, S.W.M.

AU - de Vos, W.M.

PY - 2016/12/15

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N2 - Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.

AB - Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.

M3 - Patent

M1 - WO2016198361

ER -

van der Oost J, Daas MJA, Kengen SWM, de Vos WM, inventors. Thermostable Cas9 nucleases. WO2016198361. 2016 Dec 15.