Thermostable Cas9 nucleases

J. van der Oost; M.J.A. Daas; S.W.M. Kengen; W.M. de Vos

Thermostable Cas9 nucleases

J. van der Oost (Inventor), M.J.A. Daas (Inventor), S.W.M. Kengen (Inventor), W.M. de Vos (Inventor)

Research output: Patent

Abstract

Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.

Original language	English
Patent number	WO2016198361
Priority date	12/06/15
Publication status	Published - 15 Dec 2016

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https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2016198361A1&KC=A1&FT=D&ND=3&date=20161215&DB=EPODOC&locale=en_EP

1 Patent

Thermostable Cas9 nucleases
van der Oost, J. (Inventor), van Kranenburg, R. (Inventor), Bosma, E. F. (Inventor), Mougiakos, I. (Inventor) & Mohanraju, P. (Inventor), 21 Jun 2018, Patent No. WO2018108339, Priority date 14 Dec 2016
Research output: Patent

Open Access

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title = "Thermostable Cas9 nucleases",

abstract = "Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.",

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T1 - Thermostable Cas9 nucleases

AU - van der Oost, J.

AU - Daas, M.J.A.

AU - Kengen, S.W.M.

AU - de Vos, W.M.

PY - 2016/12/15

Y1 - 2016/12/15

N2 - Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.

AB - Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.

M3 - Patent

M1 - WO2016198361

ER -

Thermostable Cas9 nucleases

Abstract

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Research output

Thermostable Cas9 nucleases

Cite this