Thermostable Cas9 nucleases

J. van der Oost (Inventor), M.J.A. Daas (Inventor), S.W.M. Kengen (Inventor), W.M. de Vos (Inventor)

Research output: Patent


Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
Original languageEnglish
Patent numberWO2016198361
Priority date12/06/15
Publication statusPublished - 15 Dec 2016


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  • Thermostable Cas9 nucleases

    van der Oost, J., van Kranenburg, R., Bosma, E. F., Mougiakos, I. & Mohanraju, P., 21 Jun 2018, Patent No. WO2018108339, Priority date 14 Dec 2016

    Research output: Patent

    Open Access

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