Theoretical modeling of the kinetics of fibrilar aggregation of bovine beta-lactoglobulin at pH 2

L.N. Arnaudov, R.J. de Vries

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23 Citations (Scopus)

Abstract

The authors propose a kinetic model for the heat-induced fibrilar aggregation of bovine ß-lactoglobulin at pH 2.0. The model involves a nucleation step and a simple addition reaction for the growth of the fibrils, as well as a side reaction leading to the irreversible denaturation and inactivation of a part of the protein molecules. For the early stages of the aggregation reaction, the authors obtain an analytical solution of the model. In agreement with their experimental results, the model predicts a critical protein concentration below where almost no fibrils are formed. The model agrees well with their experimental data from in situ light scattering. By fitting the experimental data with the model, the authors obtain the ionic strength dependent kinetic rate constants for ß-lactoglobulin fibrilar aggregation and the size of the critical nucleus.
Original languageEnglish
Article number145106
Number of pages7
JournalJournal of Chemical Physics
Volume126
Issue number14
DOIs
Publication statusPublished - 2007

Keywords

  • heat-induced denaturation
  • scattering
  • protein

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