The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus

Jordi F. Pelkmans, Aurin M. Vos, Karin Scholtmeijer, Ed Hendrix, Johan J.P. Baars, Thies Gehrmann, Marcel J.T. Reinders, Luis G. Lugones, Han A.B. Wösten

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

Original languageEnglish
Number of pages9
JournalApplied Microbiology and Biotechnology
DOIs
Publication statusPublished - 21 May 2016

Fingerprint

Agaricus
Agaricales
Schizophyllum
Basidiomycota
Agrobacterium
Zinc Fingers
Biomass
Breeding
Genes
Proteins

Keywords

  • Agaricus bisporus
  • Basidiomycete
  • Cys2His2
  • Fungi
  • Mushroom
  • Transcription factor

Cite this

Pelkmans, Jordi F. ; Vos, Aurin M. ; Scholtmeijer, Karin ; Hendrix, Ed ; Baars, Johan J.P. ; Gehrmann, Thies ; Reinders, Marcel J.T. ; Lugones, Luis G. ; Wösten, Han A.B. / The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus. In: Applied Microbiology and Biotechnology. 2016.
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abstract = "The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.",
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The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus. / Pelkmans, Jordi F.; Vos, Aurin M.; Scholtmeijer, Karin; Hendrix, Ed; Baars, Johan J.P.; Gehrmann, Thies; Reinders, Marcel J.T.; Lugones, Luis G.; Wösten, Han A.B.

In: Applied Microbiology and Biotechnology, 21.05.2016.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Pelkmans, Jordi F.

AU - Vos, Aurin M.

AU - Scholtmeijer, Karin

AU - Hendrix, Ed

AU - Baars, Johan J.P.

AU - Gehrmann, Thies

AU - Reinders, Marcel J.T.

AU - Lugones, Luis G.

AU - Wösten, Han A.B.

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AB - The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

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KW - Basidiomycete

KW - Cys2His2

KW - Fungi

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