The transcriptional architecture of the trade-off between lifespan and offspring

L.B. Snoek, A. Viñuela Rodriguez, E.W. Gutteling, A. Doroszuk, J.A.G. Riksen, J.E. Kammenga

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

The cost of reproduction, i.e. a decreased lifespan due to reproduction, is a crucial trade-off which drives life-history evolution in nearly all species, including humans. A key question in evolutionary and aging research is how this trade-off is regulated in natural populations. Although some induced mutations affecting lifespan also alter the reproductive state, detailed knowledge about the transcriptional architecture of the trade-off between lifespan and offspring is scant. We combined life-history analysis and mapping of juvenile gene transcripts in a population of recombinant inbred lines (RILs) obtained from a cross between C. elegans wildtypes N2 and CB4856. We found a trade-off between lifespan and offspring across the lines. Genetic mapping of gene expression (eQTL) revealed that the number of regulated genes increased by 20% when they were highly correlated with both traits. RNAi treatment of a few of these genes in N2 affected both offspring and lifespan. We highlight a specific case, where unc-51 RNAi increased offspring with 30% while decreasing lifespan with 15%. By introgressing a trans-acting CB4856 locus into N2 we show that the RNAi effect on the trade-off was modulated. We demonstrate that the trade-off between lifespan and offspring is highly regulated early in life and suggest that autophagic processes tune C.elegans life-history by allocating resources between offspring and lifespan.
Original languageEnglish
Title of host publicationAbstracts ESEB, 24-29 September 2009, Torino, Italy
Place of PublicationTorino
PublisherESEB
Pages10-60 Poster
Publication statusPublished - 2009
Event12th Congress European Society for Evolutionary Biology -
Duration: 24 Sep 200929 Sep 2009

Conference

Conference12th Congress European Society for Evolutionary Biology
Period24/09/0929/09/09

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Snoek, L. B., Viñuela Rodriguez, A., Gutteling, E. W., Doroszuk, A., Riksen, J. A. G., & Kammenga, J. E. (2009). The transcriptional architecture of the trade-off between lifespan and offspring. In Abstracts ESEB, 24-29 September 2009, Torino, Italy (pp. 10-60 Poster). Torino: ESEB.