The tomato phosphatidylinositol-phospholipase C2 (SlPLC2) is required for defense gene induction by the fungal elicitor xylanase

G. Gonorazky, L. Ramirez, A. Abd-El-Haliem, J.H. Vossen, L. Lamattina, A. ten Have, M.H.A.J. Joosten, A.M. Laxalt

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Abstract

The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated upon pathogen attack. We have previously shown that the fungal elicitor xylanase rapidly induces nitric oxide (NO), which is required for PI-PLCs activity and downstream defense responses in tomato cell suspensions. Here, we show that all six SlPLC genes are expressed in tomato cell suspensions. Treatment of the cells with xylanase induces an early increase in SlPLC5 transcript levels, followed by a raise of the amount of SlPLC2 transcripts. The production of NO is required to augment SlPLC5 transcript levels in xylanase-treated tomato cells. Xylanase also induces SlPLC2 and SlPLC5 transcript levels in planta. We knocked-down the expression of SlPLC2 and SlPLC5 by virus-induced gene silencing. We found that SlPLC2 is required for xylanase-induced expression of the defense-related genes PR1 and HSR203J.
Original languageEnglish
Pages (from-to)959-965
JournalJournal of Plant Physiology
Volume171
Issue number11
DOIs
Publication statusPublished - 2014

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Fungal Genes
Phospholipases
gene induction
phosphatidylinositols
xylanases
Lycopersicon esculentum
Phosphatidylinositols
tomatoes
cell suspension culture
nitric oxide
Suspensions
Nitric Oxide
Genes
genes
phospholipase C
Solanum lycopersicum
Type C Phospholipases
Gene Silencing
gene silencing
Plantae

Keywords

  • phosphatidic-acid accumulation
  • cultured rice cells
  • nitric-oxide
  • disease resistance
  • c/diacylglycerol kinase
  • arabidopsis-thaliana
  • signaling pathways
  • activation
  • responses
  • plants

Cite this

Gonorazky, G. ; Ramirez, L. ; Abd-El-Haliem, A. ; Vossen, J.H. ; Lamattina, L. ; ten Have, A. ; Joosten, M.H.A.J. ; Laxalt, A.M. / The tomato phosphatidylinositol-phospholipase C2 (SlPLC2) is required for defense gene induction by the fungal elicitor xylanase. In: Journal of Plant Physiology. 2014 ; Vol. 171, No. 11. pp. 959-965.
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abstract = "The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated upon pathogen attack. We have previously shown that the fungal elicitor xylanase rapidly induces nitric oxide (NO), which is required for PI-PLCs activity and downstream defense responses in tomato cell suspensions. Here, we show that all six SlPLC genes are expressed in tomato cell suspensions. Treatment of the cells with xylanase induces an early increase in SlPLC5 transcript levels, followed by a raise of the amount of SlPLC2 transcripts. The production of NO is required to augment SlPLC5 transcript levels in xylanase-treated tomato cells. Xylanase also induces SlPLC2 and SlPLC5 transcript levels in planta. We knocked-down the expression of SlPLC2 and SlPLC5 by virus-induced gene silencing. We found that SlPLC2 is required for xylanase-induced expression of the defense-related genes PR1 and HSR203J.",
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The tomato phosphatidylinositol-phospholipase C2 (SlPLC2) is required for defense gene induction by the fungal elicitor xylanase. / Gonorazky, G.; Ramirez, L.; Abd-El-Haliem, A.; Vossen, J.H.; Lamattina, L.; ten Have, A.; Joosten, M.H.A.J.; Laxalt, A.M.

In: Journal of Plant Physiology, Vol. 171, No. 11, 2014, p. 959-965.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Gonorazky, G.

AU - Ramirez, L.

AU - Abd-El-Haliem, A.

AU - Vossen, J.H.

AU - Lamattina, L.

AU - ten Have, A.

AU - Joosten, M.H.A.J.

AU - Laxalt, A.M.

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AB - The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipase C (PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated upon pathogen attack. We have previously shown that the fungal elicitor xylanase rapidly induces nitric oxide (NO), which is required for PI-PLCs activity and downstream defense responses in tomato cell suspensions. Here, we show that all six SlPLC genes are expressed in tomato cell suspensions. Treatment of the cells with xylanase induces an early increase in SlPLC5 transcript levels, followed by a raise of the amount of SlPLC2 transcripts. The production of NO is required to augment SlPLC5 transcript levels in xylanase-treated tomato cells. Xylanase also induces SlPLC2 and SlPLC5 transcript levels in planta. We knocked-down the expression of SlPLC2 and SlPLC5 by virus-induced gene silencing. We found that SlPLC2 is required for xylanase-induced expression of the defense-related genes PR1 and HSR203J.

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KW - responses

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