The TIR Homologue Lies near Resistance Genes in Staphylococcus aureus, Coupling Modulation of Virulence and Antimicrobial Susceptibility

Sabine Patot, Paul R.C. Imbert, Jessica Baude, Patricia Martins Simões, Jean-Baptiste Campergue, Arthur Louche, Reindert Nijland, Michèle Bès, Anne Tristan, Frédéric Laurent, Adrien Fischer, Jacques Schrenzel, François Vandenesch, Suzana P. Salcedo, Patrice François, Gérard Lina, Alice Prince (Editor)

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Toll/interleukin-1 receptor (TIR) domains in Toll-like receptors are essential for initiating and propagating the eukaryotic innate immune signaling cascade. Here, we investigate TirS, a taphylococcus aureus TIR mimic that is part of a novel bacterial invasion mechanism. Its ectopic expression in eukaryotic cells inhibited TLR signaling, downregulating the NF-kB pathway through inhibition of TLR2, TLR4, TLR5, and TLR9. Skin lesions induced by the S.
aureus knockout tirS mutant increased in a mouse model compared with wild-type and restored strains even though the tirS-mutant and wild-type strains did not differ in bacterial load. TirS also was associated with lower neutrophil and macrophage activity, confirming a central role in virulence attenuation through local inflammatory responses. TirS invariably localizes within the staphylococcal chromosomal cassettes (SCC) containing the fusC gene for fusidic acid resistance but not always carrying the mecA gene. Of note, sub-inhibitory
concentration of fusidic acid increased tirS expression. Epidemiological studies identified no link between this effector and clinical presentation but showed a selective advantage with a SCCmec element with SCC fusC/tirS. Thus, two key traits determining the success and spread of bacterial infections are linked.
Original languageEnglish
Article numbere1006092
JournalPLoS Pathogens
Volume13
Issue number1
DOIs
Publication statusPublished - 2017

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Fusidic Acid
Interleukin-1 Receptors
Virulence
Staphylococcus aureus
Bacterial Load
NF-kappa B
Toll-Like Receptors
Eukaryotic Cells
Bacterial Infections
Genes
Epidemiologic Studies
Neutrophils
Down-Regulation
Macrophages
Skin
Ectopic Gene Expression

Cite this

Patot, S., Imbert, P. R. C., Baude, J., Martins Simões, P., Campergue, J-B., Louche, A., ... Prince, A. (Ed.) (2017). The TIR Homologue Lies near Resistance Genes in Staphylococcus aureus, Coupling Modulation of Virulence and Antimicrobial Susceptibility. PLoS Pathogens, 13(1), [e1006092]. https://doi.org/10.1371/journal.ppat.1006092
Patot, Sabine ; Imbert, Paul R.C. ; Baude, Jessica ; Martins Simões, Patricia ; Campergue, Jean-Baptiste ; Louche, Arthur ; Nijland, Reindert ; Bès, Michèle ; Tristan, Anne ; Laurent, Frédéric ; Fischer, Adrien ; Schrenzel, Jacques ; Vandenesch, François ; Salcedo, Suzana P. ; François, Patrice ; Lina, Gérard ; Prince, Alice (Editor). / The TIR Homologue Lies near Resistance Genes in Staphylococcus aureus, Coupling Modulation of Virulence and Antimicrobial Susceptibility. In: PLoS Pathogens. 2017 ; Vol. 13, No. 1.
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title = "The TIR Homologue Lies near Resistance Genes in Staphylococcus aureus, Coupling Modulation of Virulence and Antimicrobial Susceptibility",
abstract = "Toll/interleukin-1 receptor (TIR) domains in Toll-like receptors are essential for initiating and propagating the eukaryotic innate immune signaling cascade. Here, we investigate TirS, a taphylococcus aureus TIR mimic that is part of a novel bacterial invasion mechanism. Its ectopic expression in eukaryotic cells inhibited TLR signaling, downregulating the NF-kB pathway through inhibition of TLR2, TLR4, TLR5, and TLR9. Skin lesions induced by the S.aureus knockout tirS mutant increased in a mouse model compared with wild-type and restored strains even though the tirS-mutant and wild-type strains did not differ in bacterial load. TirS also was associated with lower neutrophil and macrophage activity, confirming a central role in virulence attenuation through local inflammatory responses. TirS invariably localizes within the staphylococcal chromosomal cassettes (SCC) containing the fusC gene for fusidic acid resistance but not always carrying the mecA gene. Of note, sub-inhibitoryconcentration of fusidic acid increased tirS expression. Epidemiological studies identified no link between this effector and clinical presentation but showed a selective advantage with a SCCmec element with SCC fusC/tirS. Thus, two key traits determining the success and spread of bacterial infections are linked.",
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Patot, S, Imbert, PRC, Baude, J, Martins Simões, P, Campergue, J-B, Louche, A, Nijland, R, Bès, M, Tristan, A, Laurent, F, Fischer, A, Schrenzel, J, Vandenesch, F, Salcedo, SP, François, P, Lina, G & Prince, A (ed.) 2017, 'The TIR Homologue Lies near Resistance Genes in Staphylococcus aureus, Coupling Modulation of Virulence and Antimicrobial Susceptibility' PLoS Pathogens, vol. 13, no. 1, e1006092. https://doi.org/10.1371/journal.ppat.1006092

The TIR Homologue Lies near Resistance Genes in Staphylococcus aureus, Coupling Modulation of Virulence and Antimicrobial Susceptibility. / Patot, Sabine; Imbert, Paul R.C.; Baude, Jessica; Martins Simões, Patricia; Campergue, Jean-Baptiste; Louche, Arthur; Nijland, Reindert; Bès, Michèle; Tristan, Anne; Laurent, Frédéric; Fischer, Adrien; Schrenzel, Jacques; Vandenesch, François; Salcedo, Suzana P.; François, Patrice; Lina, Gérard; Prince, Alice (Editor).

In: PLoS Pathogens, Vol. 13, No. 1, e1006092, 2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The TIR Homologue Lies near Resistance Genes in Staphylococcus aureus, Coupling Modulation of Virulence and Antimicrobial Susceptibility

AU - Patot, Sabine

AU - Imbert, Paul R.C.

AU - Baude, Jessica

AU - Martins Simões, Patricia

AU - Campergue, Jean-Baptiste

AU - Louche, Arthur

AU - Nijland, Reindert

AU - Bès, Michèle

AU - Tristan, Anne

AU - Laurent, Frédéric

AU - Fischer, Adrien

AU - Schrenzel, Jacques

AU - Vandenesch, François

AU - Salcedo, Suzana P.

AU - François, Patrice

AU - Lina, Gérard

A2 - Prince, Alice

PY - 2017

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N2 - Toll/interleukin-1 receptor (TIR) domains in Toll-like receptors are essential for initiating and propagating the eukaryotic innate immune signaling cascade. Here, we investigate TirS, a taphylococcus aureus TIR mimic that is part of a novel bacterial invasion mechanism. Its ectopic expression in eukaryotic cells inhibited TLR signaling, downregulating the NF-kB pathway through inhibition of TLR2, TLR4, TLR5, and TLR9. Skin lesions induced by the S.aureus knockout tirS mutant increased in a mouse model compared with wild-type and restored strains even though the tirS-mutant and wild-type strains did not differ in bacterial load. TirS also was associated with lower neutrophil and macrophage activity, confirming a central role in virulence attenuation through local inflammatory responses. TirS invariably localizes within the staphylococcal chromosomal cassettes (SCC) containing the fusC gene for fusidic acid resistance but not always carrying the mecA gene. Of note, sub-inhibitoryconcentration of fusidic acid increased tirS expression. Epidemiological studies identified no link between this effector and clinical presentation but showed a selective advantage with a SCCmec element with SCC fusC/tirS. Thus, two key traits determining the success and spread of bacterial infections are linked.

AB - Toll/interleukin-1 receptor (TIR) domains in Toll-like receptors are essential for initiating and propagating the eukaryotic innate immune signaling cascade. Here, we investigate TirS, a taphylococcus aureus TIR mimic that is part of a novel bacterial invasion mechanism. Its ectopic expression in eukaryotic cells inhibited TLR signaling, downregulating the NF-kB pathway through inhibition of TLR2, TLR4, TLR5, and TLR9. Skin lesions induced by the S.aureus knockout tirS mutant increased in a mouse model compared with wild-type and restored strains even though the tirS-mutant and wild-type strains did not differ in bacterial load. TirS also was associated with lower neutrophil and macrophage activity, confirming a central role in virulence attenuation through local inflammatory responses. TirS invariably localizes within the staphylococcal chromosomal cassettes (SCC) containing the fusC gene for fusidic acid resistance but not always carrying the mecA gene. Of note, sub-inhibitoryconcentration of fusidic acid increased tirS expression. Epidemiological studies identified no link between this effector and clinical presentation but showed a selective advantage with a SCCmec element with SCC fusC/tirS. Thus, two key traits determining the success and spread of bacterial infections are linked.

U2 - 10.1371/journal.ppat.1006092

DO - 10.1371/journal.ppat.1006092

M3 - Article

VL - 13

JO - PLoS Pathogens

JF - PLoS Pathogens

SN - 1553-7366

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