The shoot apical meristem restores its symplasmic organization during chilling-induced release from dormancy

P.L.H. Rinne, P.M. Kaikuranta, C. van der Schoot

    Research output: Contribution to journalArticleAcademicpeer-review

    186 Citations (Scopus)

    Abstract

    The shoot apex of overwintering perennials ceases its morphogenetic activity at the end of the growing season and transforms into a bud which is dormant and freezing-tolerant. In birch (Betula pubescens) these events are triggered by short photoperiod, and involve the production of 1,3-β-D-glucan containing sphincters on the plasmodesmata. As a result, all symplasmic pathways shut down. Here we show that breakage of bud dormancy by chilling involves restoration of the symplasmic organization of the meristem. This restoration is likely to be mediated by 1,3-β-D-glucanase, which was present in small spherosome-like vacuoles that arose de novo during dormancy induction. During chilling these vacuoles were displaced from the bulk cytoplasm to the cortical cytoplasm where they became aligned with the plasma membrane, often associated with plasmodesmata. At this stage the enzyme also appeared outside the vacuoles. During chilling, 1,3-β-D-glucan disappeared from the plasmodesmal channels and wall sleeves, and the plasmodesmata regained the capacity for cell-cell transport, as demonstrated by microinjection of Lucifer Yellow CH and Fluorescein-tagged gibberellic acid. Collectively, the present experiments demonstrate that restoration of the symplasmic organization of the meristem is indispensable for the release of buds from dormancy and the assumption of a proliferation-competent state, and implicate 1,3-β-D-glucanase action at the plasmodesmata. Based on these findings we propose a model for 'dormancy cycling' which depicts the meristem as passing through three sequential states of cellular communication with characteristic sensitivities to distinct environmental cues.
    Original languageEnglish
    Pages (from-to)249-264
    JournalThe Plant Journal
    Volume26
    Issue number3
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Plasmodesmata
    plasmodesmata
    Meristem
    shoot meristems
    apical meristems
    dormancy
    Vacuoles
    meristems
    vacuoles
    Betula
    buds
    glucans
    Cytoplasm
    cytoplasm
    Betula pubescens
    sphincters
    lipid bodies
    Photoperiod
    Microinjections
    fluorescein

    Cite this

    Rinne, P.L.H. ; Kaikuranta, P.M. ; van der Schoot, C. / The shoot apical meristem restores its symplasmic organization during chilling-induced release from dormancy. In: The Plant Journal. 2001 ; Vol. 26, No. 3. pp. 249-264.
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    abstract = "The shoot apex of overwintering perennials ceases its morphogenetic activity at the end of the growing season and transforms into a bud which is dormant and freezing-tolerant. In birch (Betula pubescens) these events are triggered by short photoperiod, and involve the production of 1,3-β-D-glucan containing sphincters on the plasmodesmata. As a result, all symplasmic pathways shut down. Here we show that breakage of bud dormancy by chilling involves restoration of the symplasmic organization of the meristem. This restoration is likely to be mediated by 1,3-β-D-glucanase, which was present in small spherosome-like vacuoles that arose de novo during dormancy induction. During chilling these vacuoles were displaced from the bulk cytoplasm to the cortical cytoplasm where they became aligned with the plasma membrane, often associated with plasmodesmata. At this stage the enzyme also appeared outside the vacuoles. During chilling, 1,3-β-D-glucan disappeared from the plasmodesmal channels and wall sleeves, and the plasmodesmata regained the capacity for cell-cell transport, as demonstrated by microinjection of Lucifer Yellow CH and Fluorescein-tagged gibberellic acid. Collectively, the present experiments demonstrate that restoration of the symplasmic organization of the meristem is indispensable for the release of buds from dormancy and the assumption of a proliferation-competent state, and implicate 1,3-β-D-glucanase action at the plasmodesmata. Based on these findings we propose a model for 'dormancy cycling' which depicts the meristem as passing through three sequential states of cellular communication with characteristic sensitivities to distinct environmental cues.",
    author = "P.L.H. Rinne and P.M. Kaikuranta and {van der Schoot}, C.",
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    language = "English",
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    The shoot apical meristem restores its symplasmic organization during chilling-induced release from dormancy. / Rinne, P.L.H.; Kaikuranta, P.M.; van der Schoot, C.

    In: The Plant Journal, Vol. 26, No. 3, 2001, p. 249-264.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - The shoot apical meristem restores its symplasmic organization during chilling-induced release from dormancy

    AU - Rinne, P.L.H.

    AU - Kaikuranta, P.M.

    AU - van der Schoot, C.

    PY - 2001

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    JF - The Plant Journal

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