The Selectivity of Milking of Dunaliella salina

D.M.M. Kleinegris, M.G.J. Janssen, W.A. Brandenburg, R.H. Wijffels

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

The process of the simultaneous production and extraction of carotenoids, milking, of Dunaliella salina was studied. We would like to know the selectivity of this process. Could all the carotenoids produced be extracted? And would it be possible to vary the profile of the produced carotenoids and, consequently, influence the type of carotenoids extracted? By using three different D. salina strains and three different stress conditions, we varied the profiles of the carotenoids produced. Between Dunaliella bardawil and D. salina 19/18, no remarkable differences were seen in the extraction profiles, although D. salina 19/18 seemed to be better extractable. D. salina 19/25 was not “milkable” at all. The milking process could only be called selective for secondary carotenoids in case gentle mixing was used. In aerated flat-panel photobioreactors, extraction was much better, but selectiveness decreased and also chlorophyll and primary carotenoids were extracted. This was possibly related to cell damage due to shear stress
Original languageEnglish
Pages (from-to)14-23
JournalMarine Biotechnology
Volume12
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • beta-carotene production
  • 2-phase bioreactors
  • low-temperature
  • photosystem-ii
  • green-algae
  • accumulation
  • bardawil
  • chlorophyll
  • extraction
  • protein

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