The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

Pierre Jacob; Gwilherm  Brisou; Marion Dalmais; Johanne Thévenin; Froukje van der Wal; David Latrasse; Ravi Suresh Devani; Moussa Benhamed; Bertrand Dubreucq; Adnane Boualem; Loic Lepiniec; Richard G.H. Immink; Heribert Hirt; Abdelhafid Bendahmane

doi:10.3390/genes12050746

The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

Pierre Jacob, Gwilherm Brisou, Marion Dalmais, Johanne Thévenin, Froukje van der Wal, David Latrasse, Ravi Suresh Devani, Moussa Benhamed, Bertrand Dubreucq, Adnane Boualem, Loic Lepiniec, Richard G.H. Immink, Heribert Hirt, Abdelhafid Bendahmane^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1)
and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.

Original language	English
Article number	746
Number of pages	18
Journal	Genes
Volume	12
Issue number	5
DOIs	https://doi.org/10.3390/genes12050746
Publication status	Published - 15 May 2021

Keywords

Environmental stress
HSFA2
Seed development
Site II element
TT2/ MYB5-MBW complex

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https://edepot.wur.nl/547380Licence: CC BY

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title = "The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis",

abstract = "HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1)and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.",

keywords = "Environmental stress, HSFA2, Seed development, Site II element, TT2/ MYB5-MBW complex",

author = "Pierre Jacob and Gwilherm Brisou and Marion Dalmais and Johanne Th{\'e}venin and {van der Wal}, Froukje and David Latrasse and {Suresh Devani}, Ravi and Moussa Benhamed and Bertrand Dubreucq and Adnane Boualem and Loic Lepiniec and Immink, {Richard G.H.} and Heribert Hirt and Abdelhafid Bendahmane",

year = "2021",

month = may,

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doi = "10.3390/genes12050746",

language = "English",

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T1 - The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

AU - Jacob, Pierre

AU - Brisou, Gwilherm

AU - Dalmais, Marion

AU - Thévenin, Johanne

AU - van der Wal, Froukje

AU - Latrasse, David

AU - Suresh Devani, Ravi

AU - Benhamed, Moussa

AU - Dubreucq, Bertrand

AU - Boualem, Adnane

AU - Lepiniec, Loic

AU - Immink, Richard G.H.

AU - Hirt, Heribert

AU - Bendahmane, Abdelhafid

PY - 2021/5/15

Y1 - 2021/5/15

N2 - HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1)and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.

AB - HEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1)and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.

KW - Environmental stress

KW - HSFA2

KW - Seed development

KW - Site II element

KW - TT2/ MYB5-MBW complex

U2 - 10.3390/genes12050746

DO - 10.3390/genes12050746

M3 - Article

SN - 2073-4425

VL - 12

JO - Genes

JF - Genes

IS - 5

M1 - 746

ER -

The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis

Abstract

Keywords

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