The role of phospholipid signalling in the defence of plants against pathogens

J.H. Vossen, A. Abd-El-Haliem, W.I.L. Tameling, M.H.A.J. Joosten

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

Plants are sessile organisms and need to respond quickly to environmental stresses. The innate immune system of plants allows sensing the presence of pathogens, resulting in a defence response that prevents infection. Elicitors produced by the pathogen are specifically recognized by host-encoded receptorlike resistance (R) proteins. The R_protein Cf-4 from tomato is responsible for the recognition of the Avr4 protein elicitor produced by the leaf mold fungus Cladosporium fulvum. The receptor-like part of the Cf-4 protein, consisting of leucine rich repeats, is on the outside of the cell (where it can mediate recognition of Avr4), whereas the remainder, a short C-terminal stretch of amino acids, is inside the cell (where it is thought to initiate a defence response). Our group studies the early signalling processes that are initiated by Cf-4 inside the cell after Avr4 recognition. It was found that a very early response of Cf-4-expressing cells (within five minutes after exposure to Avr4) is the activation of two enzymes involved in phospholipid signalling; phospholipase-C (PLC) and diacylglycerol kinase (DGK). As a result of this activation, phosphatidic acid (PA) rapidly accumulates which could act as a second messenger able to initiate responses further downstream. We revealed that PLC and DGK are required for an effective defence response to C. fulvum. Accordingly, we aim to identify and characterise the PLC and DGK gene families of tomato. The identified genes will be individually silenced or over-expressed and the effect of such manipulation on disease resistance will be studied. Once the key members of the PLC and DGK families playing a role in disease resistance have been identified, we will study both the mechanism by which the encoded enzymes are activated and their role in phospholipid signalling upon pathogen recognition
Original languageEnglish
Title of host publicationBook of Abstracts XIII International Congress on Molecular Plant-Microbe Interactions, Sorrento, Italy, 21-27 July 2007
Pages96
Publication statusPublished - 2007
EventXIII International Congress on Molecular Plant-Microbe Interactions -
Duration: 21 Jul 200727 Jul 2007

Conference

ConferenceXIII International Congress on Molecular Plant-Microbe Interactions
Period21/07/0727/07/07

Fingerprint

phospholipase C
diacylglycerol kinase
plant pathogens
phospholipids
Passalora fulva
disease resistance
pathogens
proteins
tomatoes
cells
family relations
second messengers
enzymes
molds (fungi)
leucine
genes
receptors
amino acids
fungi
acids

Cite this

Vossen, J. H., Abd-El-Haliem, A., Tameling, W. I. L., & Joosten, M. H. A. J. (2007). The role of phospholipid signalling in the defence of plants against pathogens. In Book of Abstracts XIII International Congress on Molecular Plant-Microbe Interactions, Sorrento, Italy, 21-27 July 2007 (pp. 96)
Vossen, J.H. ; Abd-El-Haliem, A. ; Tameling, W.I.L. ; Joosten, M.H.A.J. / The role of phospholipid signalling in the defence of plants against pathogens. Book of Abstracts XIII International Congress on Molecular Plant-Microbe Interactions, Sorrento, Italy, 21-27 July 2007. 2007. pp. 96
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abstract = "Plants are sessile organisms and need to respond quickly to environmental stresses. The innate immune system of plants allows sensing the presence of pathogens, resulting in a defence response that prevents infection. Elicitors produced by the pathogen are specifically recognized by host-encoded receptorlike resistance (R) proteins. The R_protein Cf-4 from tomato is responsible for the recognition of the Avr4 protein elicitor produced by the leaf mold fungus Cladosporium fulvum. The receptor-like part of the Cf-4 protein, consisting of leucine rich repeats, is on the outside of the cell (where it can mediate recognition of Avr4), whereas the remainder, a short C-terminal stretch of amino acids, is inside the cell (where it is thought to initiate a defence response). Our group studies the early signalling processes that are initiated by Cf-4 inside the cell after Avr4 recognition. It was found that a very early response of Cf-4-expressing cells (within five minutes after exposure to Avr4) is the activation of two enzymes involved in phospholipid signalling; phospholipase-C (PLC) and diacylglycerol kinase (DGK). As a result of this activation, phosphatidic acid (PA) rapidly accumulates which could act as a second messenger able to initiate responses further downstream. We revealed that PLC and DGK are required for an effective defence response to C. fulvum. Accordingly, we aim to identify and characterise the PLC and DGK gene families of tomato. The identified genes will be individually silenced or over-expressed and the effect of such manipulation on disease resistance will be studied. Once the key members of the PLC and DGK families playing a role in disease resistance have been identified, we will study both the mechanism by which the encoded enzymes are activated and their role in phospholipid signalling upon pathogen recognition",
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Vossen, JH, Abd-El-Haliem, A, Tameling, WIL & Joosten, MHAJ 2007, The role of phospholipid signalling in the defence of plants against pathogens. in Book of Abstracts XIII International Congress on Molecular Plant-Microbe Interactions, Sorrento, Italy, 21-27 July 2007. pp. 96, XIII International Congress on Molecular Plant-Microbe Interactions, 21/07/07.

The role of phospholipid signalling in the defence of plants against pathogens. / Vossen, J.H.; Abd-El-Haliem, A.; Tameling, W.I.L.; Joosten, M.H.A.J.

Book of Abstracts XIII International Congress on Molecular Plant-Microbe Interactions, Sorrento, Italy, 21-27 July 2007. 2007. p. 96.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - The role of phospholipid signalling in the defence of plants against pathogens

AU - Vossen, J.H.

AU - Abd-El-Haliem, A.

AU - Tameling, W.I.L.

AU - Joosten, M.H.A.J.

PY - 2007

Y1 - 2007

N2 - Plants are sessile organisms and need to respond quickly to environmental stresses. The innate immune system of plants allows sensing the presence of pathogens, resulting in a defence response that prevents infection. Elicitors produced by the pathogen are specifically recognized by host-encoded receptorlike resistance (R) proteins. The R_protein Cf-4 from tomato is responsible for the recognition of the Avr4 protein elicitor produced by the leaf mold fungus Cladosporium fulvum. The receptor-like part of the Cf-4 protein, consisting of leucine rich repeats, is on the outside of the cell (where it can mediate recognition of Avr4), whereas the remainder, a short C-terminal stretch of amino acids, is inside the cell (where it is thought to initiate a defence response). Our group studies the early signalling processes that are initiated by Cf-4 inside the cell after Avr4 recognition. It was found that a very early response of Cf-4-expressing cells (within five minutes after exposure to Avr4) is the activation of two enzymes involved in phospholipid signalling; phospholipase-C (PLC) and diacylglycerol kinase (DGK). As a result of this activation, phosphatidic acid (PA) rapidly accumulates which could act as a second messenger able to initiate responses further downstream. We revealed that PLC and DGK are required for an effective defence response to C. fulvum. Accordingly, we aim to identify and characterise the PLC and DGK gene families of tomato. The identified genes will be individually silenced or over-expressed and the effect of such manipulation on disease resistance will be studied. Once the key members of the PLC and DGK families playing a role in disease resistance have been identified, we will study both the mechanism by which the encoded enzymes are activated and their role in phospholipid signalling upon pathogen recognition

AB - Plants are sessile organisms and need to respond quickly to environmental stresses. The innate immune system of plants allows sensing the presence of pathogens, resulting in a defence response that prevents infection. Elicitors produced by the pathogen are specifically recognized by host-encoded receptorlike resistance (R) proteins. The R_protein Cf-4 from tomato is responsible for the recognition of the Avr4 protein elicitor produced by the leaf mold fungus Cladosporium fulvum. The receptor-like part of the Cf-4 protein, consisting of leucine rich repeats, is on the outside of the cell (where it can mediate recognition of Avr4), whereas the remainder, a short C-terminal stretch of amino acids, is inside the cell (where it is thought to initiate a defence response). Our group studies the early signalling processes that are initiated by Cf-4 inside the cell after Avr4 recognition. It was found that a very early response of Cf-4-expressing cells (within five minutes after exposure to Avr4) is the activation of two enzymes involved in phospholipid signalling; phospholipase-C (PLC) and diacylglycerol kinase (DGK). As a result of this activation, phosphatidic acid (PA) rapidly accumulates which could act as a second messenger able to initiate responses further downstream. We revealed that PLC and DGK are required for an effective defence response to C. fulvum. Accordingly, we aim to identify and characterise the PLC and DGK gene families of tomato. The identified genes will be individually silenced or over-expressed and the effect of such manipulation on disease resistance will be studied. Once the key members of the PLC and DGK families playing a role in disease resistance have been identified, we will study both the mechanism by which the encoded enzymes are activated and their role in phospholipid signalling upon pathogen recognition

M3 - Abstract

SP - 96

BT - Book of Abstracts XIII International Congress on Molecular Plant-Microbe Interactions, Sorrento, Italy, 21-27 July 2007

ER -

Vossen JH, Abd-El-Haliem A, Tameling WIL, Joosten MHAJ. The role of phospholipid signalling in the defence of plants against pathogens. In Book of Abstracts XIII International Congress on Molecular Plant-Microbe Interactions, Sorrento, Italy, 21-27 July 2007. 2007. p. 96