The RETINOBLASTOMA-RELATED gene regulates stem cell maintenance in Arabidopsis roots

Marjolein Wildwater*, Ana Campilho, Jose Manuel Perez-Perez, Renze Heidstra, Ikram Blilou, Henrie Korthout, Jayanta Chatterjee, Luisa Mariconti, Wilhelm Gruissem, Ben Scheres

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

273 Citations (Scopus)

Abstract

The maintenance of stem cells in defined locations is crucial for all multicellular organisms. Although intrinsic factors and signals for stem cell fate have been identified in several species, it has remained unclear how these connect to the ability to reenter the cell cycle that is one of the defining properties of stem cells. We show that local reduction of expression of the RETINOBLASTOMA-RELATED (RBR) gene in Arabidopsis roots increases the amount of stem cells without affecting cell cycle duration in mitotically active cells. Conversely, induced RBR overexpression dissipates stem cells prior to arresting other mitotic cells. Overexpression of D cyclins, KIP-related proteins, and E2F factors also affects root stem cell pool size, and genetic interactions suggest that these factors function in a canonical RBR pathway to regulate somatic stem cells. Expression analysis and genetic interactions position RBR-mediated regulation of the stem cell state downstream of the patterning gene SCARECROW.

Original languageEnglish
Pages (from-to)1337-1349
Number of pages13
JournalCell
Volume123
Issue number7
DOIs
Publication statusPublished - 29 Dec 2005
Externally publishedYes

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