The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing

L. Mlynarova, A. Hricova, A. Loonen, J.P.H. Nap

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous
Original languageEnglish
Pages (from-to)2203-2217
JournalThe Plant Cell
Volume15
DOIs
Publication statusPublished - 2003

Fingerprint

Glucuronidase
RNA Interference
RNA interference
Transgenes
transgenes
Chromatin
Tobacco
chromatin
tobacco
site-specific recombination
genes
lysozyme
reporter genes
genetically modified organisms
chickens
Genes
gene expression
Muramidase
Reporter Genes
Genetic Recombination

Keywords

  • matrix attachment regions
  • dna methylation
  • messenger-rna
  • domain boundaries
  • single-copy
  • gene
  • expression
  • plants
  • suppression
  • interference

Cite this

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title = "The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing",
abstract = "We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous",
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author = "L. Mlynarova and A. Hricova and A. Loonen and J.P.H. Nap",
year = "2003",
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journal = "The Plant Cell",
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publisher = "American Society of Plant Biologists",

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The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing. / Mlynarova, L.; Hricova, A.; Loonen, A.; Nap, J.P.H.

In: The Plant Cell, Vol. 15, 2003, p. 2203-2217.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing

AU - Mlynarova, L.

AU - Hricova, A.

AU - Loonen, A.

AU - Nap, J.P.H.

PY - 2003

Y1 - 2003

N2 - We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous

AB - We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous

KW - matrix attachment regions

KW - dna methylation

KW - messenger-rna

KW - domain boundaries

KW - single-copy

KW - gene

KW - expression

KW - plants

KW - suppression

KW - interference

U2 - 10.1105/tpc.012070

DO - 10.1105/tpc.012070

M3 - Article

VL - 15

SP - 2203

EP - 2217

JO - The Plant Cell

JF - The Plant Cell

SN - 1040-4651

ER -