The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing

L. Mlynarova; A. Hricova; A. Loonen; J.P.H. Nap

doi:10.1105/tpc.012070

The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing

L. Mlynarova, A. Hricova, A. Loonen, J.P.H. Nap

Laboratory of Molecular Biology

Research output: Contribution to journal › Article › Academic › peer-review

10 Citations (Scopus)

Abstract

We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous

Original language	English
Pages (from-to)	2203-2217
Journal	The Plant Cell
Volume	15
DOIs	https://doi.org/10.1105/tpc.012070
Publication status	Published - 2003

Keywords

matrix attachment regions
dna methylation
messenger-rna
domain boundaries
single-copy
gene
expression
plants
suppression
interference

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10.1105/tpc.012070

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title = "The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing",

abstract = "We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous",

keywords = "matrix attachment regions, dna methylation, messenger-rna, domain boundaries, single-copy, gene, expression, plants, suppression, interference",

author = "L. Mlynarova and A. Hricova and A. Loonen and J.P.H. Nap",

year = "2003",

doi = "10.1105/tpc.012070",

language = "English",

volume = "15",

pages = "2203--2217",

journal = "The Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

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TY - JOUR

T1 - The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing

AU - Mlynarova, L.

AU - Hricova, A.

AU - Loonen, A.

AU - Nap, J.P.H.

PY - 2003

Y1 - 2003

N2 - We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous

AB - We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous

KW - matrix attachment regions

KW - dna methylation

KW - messenger-rna

KW - domain boundaries

KW - single-copy

KW - gene

KW - expression

KW - plants

KW - suppression

KW - interference

U2 - 10.1105/tpc.012070

DO - 10.1105/tpc.012070

M3 - Article

SN - 1040-4651

VL - 15

SP - 2203

EP - 2217

JO - The Plant Cell

JF - The Plant Cell

ER -

The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing

Abstract

Keywords

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