The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing

L. Mlynarova, A. Hricova, A. Loonen, J.P.H. Nap

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous
Original languageEnglish
Pages (from-to)2203-2217
JournalThe Plant Cell
Volume15
DOIs
Publication statusPublished - 2003

Keywords

  • matrix attachment regions
  • dna methylation
  • messenger-rna
  • domain boundaries
  • single-copy
  • gene
  • expression
  • plants
  • suppression
  • interference

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