The potential of virus-induced gene silencing for speeding up functional characterization of plant genes

V.A. Benedito, P.B. Visser, G.C. Angenent, F.A. Krens

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60 Citations (Scopus)

Abstract

Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include the circumvention of plant transformation, methodological simplicity and robustness, and speedy results. These features make VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is already well established in Nicotiana benthamiana; however, efforts are being made to improve VIGS in other species, including monocots. Current research is focussed on unravelling the mechanisms of post-transcriptional gene silencing and VIGS, as well as on finding novel viral vectors in order to broaden the host species spectrum. We examined how VIGS has been used to assess gene functions in plants, including molecular mechanisms involved in the process, available methodological elements, such as vectors and inoculation procedures, and we looked for examples in which the system has been applied successfully to characterize gene function in plants.
Original languageEnglish
Pages (from-to)323-341
JournalGenetics and Molecular Research
Volume3
Issue number3
Publication statusPublished - 2004

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Plant Genes
Gene Silencing
Viruses
Reverse Genetics
DNA Transposable Elements
Genomics
RNA Interference
Genes
Tobacco
Research

Cite this

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title = "The potential of virus-induced gene silencing for speeding up functional characterization of plant genes",
abstract = "Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include the circumvention of plant transformation, methodological simplicity and robustness, and speedy results. These features make VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is already well established in Nicotiana benthamiana; however, efforts are being made to improve VIGS in other species, including monocots. Current research is focussed on unravelling the mechanisms of post-transcriptional gene silencing and VIGS, as well as on finding novel viral vectors in order to broaden the host species spectrum. We examined how VIGS has been used to assess gene functions in plants, including molecular mechanisms involved in the process, available methodological elements, such as vectors and inoculation procedures, and we looked for examples in which the system has been applied successfully to characterize gene function in plants.",
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The potential of virus-induced gene silencing for speeding up functional characterization of plant genes. / Benedito, V.A.; Visser, P.B.; Angenent, G.C.; Krens, F.A.

In: Genetics and Molecular Research, Vol. 3, No. 3, 2004, p. 323-341.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - The potential of virus-induced gene silencing for speeding up functional characterization of plant genes

AU - Benedito, V.A.

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AB - Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include the circumvention of plant transformation, methodological simplicity and robustness, and speedy results. These features make VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is already well established in Nicotiana benthamiana; however, efforts are being made to improve VIGS in other species, including monocots. Current research is focussed on unravelling the mechanisms of post-transcriptional gene silencing and VIGS, as well as on finding novel viral vectors in order to broaden the host species spectrum. We examined how VIGS has been used to assess gene functions in plants, including molecular mechanisms involved in the process, available methodological elements, such as vectors and inoculation procedures, and we looked for examples in which the system has been applied successfully to characterize gene function in plants.

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