Insight in the molecular basis of virulence can help us to identify new and more durable sources of resistance to the root-knot nematode Meloidogyne incognita in tomato. Recently, we identified the effector MiL648 by applying a genome-wide scan for positively selected secreted proteins. To acquire a better understanding of the function of MiL648, we are currently functionally characterising the protein in plants using host-induced gene silencing. Several in planta targets of the putative effector have now been found by a yeast-two-hybrid screening and interactions were checked using Co-IPs. To identify the role of the host targets in the infection process, homologs of the host targets were knocked out in Arabidopsis.
|Publication status||Published - 2 Jul 2018|
|Event||3rd International Conference “Plant Biotic Stresses & Resistance Mechanisms" - Vienna, Austria|
Duration: 2 Jul 2018 → 3 Jul 2018
|Conference||3rd International Conference “Plant Biotic Stresses & Resistance Mechanisms"|
|Period||2/07/18 → 3/07/18|
Verhoeven, A., Put, S. (Ed.), Slootweg, E. J. (Ed.), van Raaij, D. R. (Ed.), Overmars, H. A. (Ed.), Goverse, A. (Ed.), & Smant, G. (Ed.) (2018). The positively-selected effector MiL648 of the root-knot nematode Meloidogyne incognita plays a role in tomato infections. Poster session presented at 3rd International Conference “Plant Biotic Stresses & Resistance Mechanisms", Vienna, Austria.